DESCRIPTION

• Aa: 2-332(end)
• Mw: 37.5 kDa
• Tags: N-terminal His-tag
• Format: Aqueous buffer solution
• Genbank: NM_005566
• Species: Human
• Uniprot: P00338
• Shiptemp: -80°C (dry ice)
• Synonyms: Lactate dehydrogenase A, LDHA
• Warnings: Avoid freeze/thaw cycles.
• Category: Metabolic Enzymes/Protein
• References: 1. Rong Y., et al. Tumour Biol. 2013. 34(3):1523-30.2. Tang H., et al. Biol Reprod. 2013. 88(4):96.
• Description: Human lactate dehydrogenase A (LDHA), GenBank Accession No. NM_005566), full length [2-332(end)] with N-terminal His-tag, MW= 37.5kDa, expressed in an E. coli expression system.
• Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 160 mM imidazole, 0.04% Tween-20, and 20% glycerol.
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
• Product Type: Protein
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: Reaction was conducted in 100 mM Tris, pH 7.4, 2 mM sodium pyruvate, 0.2 mM β-NADH, 0.02% BSA, and LDHA in a volume of 200 µL. Initiate reactions with LDHA. Monitor the decrease in NADH absorbance at 340 nm at 37°C for 5 min. Read at Abs340 using Tecan Infinite 1000.
• Specific Activity: ≥700 nmole/min/µg.
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Metabolic Enzymes-Dehydrogenase