Anti-Integrin β4 (Tyr-1526), Phosphospecific Antibody

DETAILS

Form: Antigen Affinity Purified
Gene: ITGB4
Host: Rabbit
Rrid: AB_2129013
Type: Primary Antibody
Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Target: Integrin β4 (Tyr-1526)
Isotype: IgG
Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Shipping: Blue Ice
Synonyms: integrin, CD104, GP150
Clonality: Rabbit Polyclonal
Immunogen: Phospho-Integrin β4 (Tyr-1526) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 1526 of human Integrin β4. This peptide sequence is found in all three Integrin β4 isoforms and is highly conserved in rat and mouse Integrin β4.
Expiration: After date of receipt, stable for at least 1 year at -20°C.
Specificity: This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose and to unphosphorylated Integrin β4 (Tyr-1526) peptide before affinity purification using phospho-Integrin β4 (Tyr-1526) peptide (without carrier). The purified antibody detects a 200kDa* protein corresponding to the molecular mass of Integrin β4 on SDS-PAGE immunoblots of A431 and src-transformed Hct116 cells treated with pervanadate, but not in control cells.
Applications: WB, ICC
Conjugate Exem: Unconjugated
Physical State: Liquid
Quality Control: Western blots performed on each lot.
Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Molecular Weight: 200
Dilution Range Wb: 1:1000
Immunogen Species: Human
Dilution Range Icc: 1:100
Species Reactivity: Human, Mouse, and Rat
Target Description: The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins. Activation of IκBα occurs through both serine and tyrosine phosphorylation events. Activation through phosphorylation at Ser-32 and Ser-36 is followed by proteasome-mediated degradation, resulting in the release and nuclear translocation of active NF-κB. This pathway of IκBα regulation occurs in response to various NF-κB-activating agents, such as TNFα, interleukins, LPS, and irradiation. An alternative pathway for IκBα regulation occurs through tyrosine phosphorylation of Tyr-42 and Tyr-305. Tyr-42 is phosphorylated in response to oxidative stress and growth factors. This phosphorylation can lead to degradation of IκBα and NF-κB-activation. In contrast, Tyr-305 phosphorylation by c-Abl has been implicated in IκBα nuclear translocation and inhibition of NF-κB-activation. Thus, tyrosine phosphorylation of IκBα may be an important regulatory mechanism in NF-κB signaling.
Uniprot Number Immunogen Species: P16144
Post Translational Modification Type: Phosphorylated
Post Translational Modification Residue: Tyr-1526