Anti-Mitofilin Antibody

DETAILS

Form: Protein G Purified
Gene: IMMT
Host: Mouse
Rrid: AB_3068465
Type: Primary Antibody
Clone: M027
Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Target: Mitofilin
Isotype: IgG1
Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Shipping: Blue Ice
Synonyms: MICOS complex subunit MIC60, Cell proliferation-inducing gene 4/52 protein,Mitochondrial inner membrane protein Mitofilin p87/89, IMMT, HMP, MIC60, MINOS2, PIG4, PIG52, MINOS
Clonality: Mouse Monoclonal
Immunogen: Clone M027 was generated from a proprietary antigen related to human mitofilin expressed in the A431 epidermoid carcinoma cell line.
Expiration: After date of receipt, stable for at least 1 year at -20°C.
Specificity: Clone M027 detects a 90 kDa* band corresponding to the molecular mass of mitofilin on SDS-PAGE immunoblots of native or denatured human PC3, MDA-MB-231, A431, A549, and MeWo cell lysates, as well as human breast, skin, and brain tissues. In addition, mass spectrometry analysis of immunoprecipitates using MM0271 in human A431 cell lysates confirms this antibody only detects Mitofilin protein. The antibody can be used for multiple applications including ELISA, western blot, immunocytochemistry, and immunoprecipitation.
Applications: WB, ICC, IP
Conjugate Exem: Unconjugated
Physical State: Liquid
Quality Control: Western blots performed on each lot.
Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Molecular Weight: 90
Dilution Range Ip: 1:100
Dilution Range Wb: 1:1000
Immunogen Species: Human
Dilution Range Icc: 1:100
Species Reactivity: Human
Target Description: Mitofilin is an important organizer of mitochondrial architecture. The mitofilin sequence encodes a polypeptide with a central alpha-helical region with predicted coiled coil domains flanked by globular amino and carboxy termini. There are four isoforms of mitofilin, and 90/91 kDa mitofilin forms have been observed in western blots. Mitofilin is located in the inner mitochondrial membrane and interacts with several protein complexes of the outer membrane, thereby generating contact sites between the two membrane systems of mitochondria. These mitofilin-containing hetero-oligomeric protein complexes form the mitochondrial inner membrane organizing system (MINOS). MINOS integrity is required for the structural maintenance of the inner mitochondrial membrane. This mitochondrial region contains cristae membranes that form large tubular invaginations that protrude into the mitochondrial matrix. These cristae membranes contain the enzyme complexes of the oxidative phosphorylation machinery. MINOS deficiency causes loss of crista junction structures and the detachment of cristae from the inner boundary membrane.
Uniprot Number Immunogen Species: Q16891
Post Translational Modification Type: GOmAb