DESCRIPTION

• Mw: 42.2 kDa
• Tag: Myc-DDK
• Accn: NM_001282016
• Type: Mouse Tagged ORF Clone
• Refseq: NM_001282016.1, NP_001268945.1
• Status: In Stock*
• Symbol: P2ry1
• Vector: pCMV6-Entry
• Category: cDNA Clones
• Locus Id: 18441
• Orf Size: 1119 bp
• Synonyms: P2Y1
• Refseq Orf: 1122 bp
• Uniprot Id: P49650
• Refseq Size: 3624 bp
• Availability: In Stock
• Cytogenetics: 3 E1
• Gene Summary: Receptor for extracellular adenine nucleotides such as ADP. In platelets, binding to ADP leads to mobilization of intracellular calcium ions via activation of phospholipase C, a change in platelet shape, and ultimately platelet aggregation.[UniProtKB/Swiss-Prot Function]
• Sequence Data: ORF Nucleotide Sequence ( show hide ) >MR229547 representing NM_001282016 Red =Cloning site Blue=ORF Green =Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGC C ATGACCGAGGTGCCTTGGTCGGTTGTCCCCAACGGGACGGATGCTGCCTTTCTGGCGGGCCTGGGCTCGC TTTGGGGAAACAGTACTGTCGCCTCAACTGCAGCAGTTTCCTCTTCATTCCAATGTGCCCTGACCAAGAC CGGTTTCCAGTTCTACTACCTGCCGGCTGTCTACATTTTAGTGTTCATCATAGGCTTCCTAGGCAACAGC GTGGCTATCTGGATGTTCGTTTTCCACATGAAGCCTTGGAGCGGCATCTCCGTGTACATGTTCAATTTGG CTCTGGCTGACTTTTTATACGTGCTCACCCTACCAGCCCTCATCTTCTACTACTTCAACAAGACTGACTG GATCTTCGGGGATGCTATGTGCAAGCTGCAGAGATTCATCTTCCACGTAAATCTCTACGGTAGCATCTTG TTCCTCACCTGCATCAGCGCACACAGGTACAGTGGCGTGGTGTACCCTCTCAAGTCTCTGGGCAGGCTCA AGAAGAAGAATGCCATTTATGTCAGCGTGCTGGTGTGGCTCATTGTGGTGGTGGCCATCTCCCCTATTCT CTTCTACTCTGGCACTGGGACTCGGAAAAACAAAACTGTCACCTGCTATGACACCACGTCCAATGATTAC CTGCGAAGTTATTTCATCTACAGTATGTGCACGACTGTGGCCATGTTCTGCATCCCTTTGGTGCTGATCT TGGGCTGTTATGGATTAATTGTTAAAGCTTTGATTTACAATGACCTGGACAACTCTCCGCTCCGGAGGAA ATCCATTTACCTGGTGATAATTGTCCTGACGGTGTTTGCTGTGTCTTATATCCCTTTCCATGTGATGAAA ACGATGAATTTGCGAGCACGGTTGGATTTCCAGACCCCAGAAATGTGTGATTTCAACGACAGGGTTTATG CCACTTATCAGGTAACAAGAGGTCTAGCAAGTCTCAACAGCTGTGTGGACCCCATTCTTTATTTCTTGGC TGGAGATACATTCAGAAGGAGACTGTCCCGAGCCACCAGGAAAGCTTCCAGGAGGAGTGAGGCCAATTTA CAATCCAAGAGTGAAGAAATGACTCTCAATATTTTATCTGAGTTCAAGCAGAATGGAGACACGAGTTTG ACGCGT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAG GTTTAA Protein Sequence ( show hide ) >MR229547 representing NM_001282016 Red =Cloning site Green =Tags(s) MTEVPWSVVPNGTDAAFLAGLGSLWGNSTVASTAAVSSSFQCALTKTGFQFYYLPAVYILVFIIGFLGNS VAIWMFVFHMKPWSGISVYMFNLALADFLYVLTLPALIFYYFNKTDWIFGDAMCKLQRFIFHVNLYGSIL FLTCISAHRYSGVVYPLKSLGRLKKKNAIYVSVLVWLIVVVAISPILFYSGTGTRKNKTVTCYDTTSNDY LRSYFIYSMCTTVAMFCIPLVLILGCYGLIVKALIYNDLDNSPLRRKSIYLVIIVLTVFAVSYIPFHVMK TMNLRARLDFQTPEMCDFNDRVYATYQVTRGLASLNSCVDPILYFLAGDTFRRRLSRATRKASRRSEANL QSKSEEMTLNILSEFKQNGDTSL TR TRPLEQKLISEEDLAANDILDYKDDDDK V
• Oti Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Oti Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• E Coli Selection: Kanamycin (25 ug/mL)
• Restriction Sites: SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Mammalian Cell Selection: Neomycin