CP2581
Anti-Coronin-1B (C-terminus) Antibody
PhosphoSolutions
DETAILS
- Form: Antigen Affinity Purified
- Gene: CORO1B
- Host: Rabbit
- Rrid: AB_2082089
- Type: Primary Antibody
- Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
- Target: Coronin-1B (C-terminus)
- Isotype: IgG
- Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
- Shipping: Blue Ice
- Synonyms: Coronin2, Coro1B
- Clonality: Rabbit Polyclonal
- Immunogen: Coronin-1B (C-terminus) synthetic peptide (coupled to carrier protein) corresponds to amino acids at the C-terminus of human coronin-1B. This sequence has high homology to the conserved site in rat and mouse coronin-1B, and less than 50% homology to similar regions in other coronins.
- Expiration: After date of receipt, stable for at least 1 year at -20°C.
- Specificity: The antibody detects a 60 kDa* band corresponding to Coronin-1B on SDS-PAGE immunoblots of human A431 and HeLa, mouse brain, and rabbit spleen fibroblasts.
- Applications: WB, ICC
- Conjugate Exem: Unconjugated
- Physical State: Liquid
- Quality Control: Western blots performed on each lot.
- Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
- Molecular Weight: 60
- Dilution Range Wb: 1:1000
- Immunogen Species: Human
- Dilution Range Icc: 1:100
- Species Reactivity: Human, Mouse, and Rat
- Target Description: Coronins are highly-conserved F-actin binding proteins that play important roles in lamellipodial protrusion during various types of cell motility. In yeast, coronins regulate cytoskeletal changes through inhibition of Arp2/3 complex. Human coronins have been classified in three subgroups type I (coronin-1A, -1B, -1C), type II (coronin-2A, -2B), and type III (coronin-7). These coronins have at least one large b-propeller region that mediates protein-protein interactions and type I and II coronins have coiled-coil regions involved in oligiomerization. Coronin-1B is ubiquitously expressed and localizes to the leading edge of cell protrusions in migrating fibroblasts. Both Coronin-1B and Coronin-1A interaction with Arp2/3 complex may be regulated by phosphorylation. PKC phosphorylates the N-terminus at Ser-2 , and this phosphorylation reduces interactions with Arp2/3 leading to diminshed cell motility.
- Uniprot Number Immunogen Species: Q9BR76