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51008

MagVigen™ - anti c-Myc, mouse

NVIGEN

DETAILS

  • Category: Purification Reagent
  • Lead Time: 1 week
  • Subcategory: Magnetic Beads
  • Applications: Protein Purification, Co-immunoprecipitation
  • Product and Related Product Contents: Cat# 51008: MagVigen™-anti-c-Myc nanoparticles are provided in phosphate buffered saline (PBS), pH 7.4. Each vial contains 1 ml of solution with a particle concentration of 1 mg/ml, which is enough for approximately 5-50 antibody enrichment or immunoprecipitation assays. Stored at 4°C up to 6 months.

DESCRIPTION

Product Description MagVigen™-anti-c-Myc nanoparticles are ideal for the purification of c-Myc tagged recombinant proteins and immunoprecipitation assays. The subsequent nanoparticle-c-Myc protein complex can be easily separated from the rest of the sample by magnet. The retained c-Myc fusion proteins can be eluted from the nanoparticles using an elution buffer. MagVigen™ nanoparticles enable identification of new protein-protein interactions through immunoprecipitation assays, where the MagVigen™ -c-Myc protein complex can be used to isolate particular proteins of interest or protein complex from assay samples, e.g. cell lysate. The immunoprecipitated proteins can be further analyzed by electrophoresis, protein staining, and mass spectrometry. MagVigen™ nanoparticles are much smaller than conventional micro-beads. This feature allows for better accessibility of the nanoparticles to the antigenic epitope and for less disturbance to the native functions of proteins or protein- protein complexes. In addition, the surfaces of MagVigen™ nanoparticles are uniquely coated to reduce non-specific interactions with cellular proteins and other biomolecules. This feature allows for a more specific “pull down” of real protein complex targets.