Extract-EZ K1, membrane and Cytoplasmic Protein Extraction Kit: Membrane and Cytoplasmic Protein Extraction Kit is designed for effective extraction of membrane and cytoplasmic proteins from mammalian tissues and culture cells. Treatment using different mild, non-denaturing detergent based buffers allows selective separation of membrane and membrane-associated proteins from the cytoplasmic fraction. The kit is simple and rapid and can efficiently isolate high quality membrane and cytoplasmic protein extracts without crosscontamination between the two fractions. Both fractions contain high quality and purity, nondenatured functional proteins which can be utilized in a variety of applications, such as Western blotting, and enzyme analyses, SDS-PAGE, IP, EMSA, Pull down and so on. The procedure is based on stepwise differential protein solubilization. The kit can be used for 50 sample preparations (from 50x100 mg tissue or 50x 107 culture cell per sample).