Anti-Girdin (Ser-1674), Phosphospecific Antibody

DETAILS

Form: Antigen Affinity Purified
Gene: CCDC88A
Host: Rabbit
Rrid: AB_3068447
Type: Primary Antibody
Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Target: Girdin (Ser-1674)
Isotype: IgG
Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Shipping: Blue Ice
Synonyms: APE, Galpha, vesicle, GIV, Girders actin filament, HkRP1, GRDN, CCDC88A
Clonality: Rabbit Polyclonal
Immunogen: Phospho-Girdin (Ser-1674) synthetic peptide (coupled to carrier) corresponding to amino acid residues around serine 1674 in human Girdin. This sequence has high homology to rat and mouse Girdin, and has low homology to similar regions in other Girdin family members.
Expiration: After date of receipt, stable for at least 1 year at -20°C.
Specificity: The antibody was cross-adsorbed to unphosphorylated Girdin (Ser-1674) peptide before affinity purification using phospho-Girdin (Ser-1674) peptide. This antibody detects a 250 kDa* protein on SDS-PAGE immunoblots of human A431 cells stimulated with Calyculin A. This reactivity is not observed after lambda phosphatase treatment to dephosphorylate Girdin.
Applications: WB
Conjugate Exem: Unconjugated
Physical State: Liquid
Quality Control: Western blots performed on each lot.
Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Molecular Weight: 250
Dilution Range Wb: 1:1000
Immunogen Species: Human
Species Reactivity: Human, Mouse, and Rat
Target Description: Girdin, a member of the CCDC88 (Hook related protein) family, is an actin binding protein involved with cell migration and maintaining cytoskeletal organization. Girdin has conserved domains at the N- and C-terminus that bind microtubules and actin, respectively. It enhances PI3-kinase dependent phosphorylation of proteins, most notably Akt. This same activity can contribute to tumor proliferation, invasion, and metastasis in breast, ovarian, prostate, and pancreatic tissues. Girdin is phosphorylated at three separate locations: Ser-1416, Ser-1674, and Tyr-1764. Ser-1416 is the primary Akt phosphorylation site, while Cyclin-dependent kinases interact with Girdin and phosphorylate Ser-1674. Multiple receptor tyrosine kinases can bind girdin and phosphorylate Tyr-1764.
Uniprot Number Immunogen Species: Q3V6T2
Post Translational Modification Type: Phosphorylated
Post Translational Modification Residue: Ser-1674