Polyclonal Antibody to STAT1 (Phospho-Ser727)

DESCRIPTION

Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-a and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, eading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state.

DETAILS

Gene: STAT1
App Id: WB,IHC,IF
Antigen: STAT1 (Phospho-Ser727)
Gene Id: 6772
Isotype: Rabbit IgG
Format Id: Purified
Uniprot Id: P42224
Host Animal: Rabbit
Clonality Id: Polyclonal
Reactivity Id: Human,Mouse
Product Content: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Alternative Name: ISGF-3|| STAT9
Application Note: Predicted MW: 91kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Storage Condition: Store the antibody at 4°C, stable for 6 months. For ong-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Immunogen Information: Peptide sequence around phosphorylation site of serine 727 (P-M-S(p)-P-E) derived from Human STAT1.