DESCRIPTION

• Aa: full length
• Mw: 42.4 kDa
• Tags: C-terminal His-tag
• Format: Aqueous buffer solution
• Genbank: NM_018486
• Species: Human
• Uniprot: Q9BY41
• Shiptemp: -80°C (dry ice)
• Synonyms: histone deacetylase 8, HDAC-8
• Warnings: Avoid freeze/thaw cycles.
• Category: Deacetylase/Enzymes
• References: 1. Lee, H. et al. Mol. Cell. Biol. 26 (14), 5259-5269 (2006).2. Gantt,S.L. et al., Biochemistry 45 (19), 6170-6178 (2006). Application References: 1. Design, synthesis, 3D pharmacophore, QSAR, and docking studies of carboxylic acid derivatives as Histone Deacetylase inhibitors and cytotoxic agents (2014) 2. Discovery of a small molecule agonist of phosphatidylinositol 3-kinase p110α that reactivates latent HIV-1 (2014) 3. Wang, C., et al. J Med Chem. 2014 Oct 9;57(19):7999-8009. doi: 10.1021/jm500872p. In Vivo Imaging of Histone Deacetylases (HDACs) in the Central Nervous System and Major Peripheral Organs (2014) 4. The effect of various zinc binding groups on inhibition of histone deacetylases 1-11 (2014) 5. Meyners, C., et al. Mol Recognit. 2014 Mar;27(3):160-72. Thermodynamics of ligand binding to histone deacetylase like amidohydrolase from Bordetella/Alcaligenes (2014) 6. A selective HDAC 1/2 inhibitor modulates chromatin and gene expression in brain and alters mouse behavior in two mood-related tests (2013) 7. Fass, D.M., et al. Neuropharmacology. 2013 Jan; 64: 81–96. Crebinostat: a novel cognitive enhancer that inhibits histone deacetylase activity and modulates chromatin-mediated neuroplasticity (2013) 8. FDG-PET imaging reveals local brain glucose utilization is altered by class I histone deacetylase inhibitors (2013) 9. Baud, MGJ, et al. ChemMedChem. 2013 Jan;8(1):149-56. doi: 10.1002/cmdc.201200450. Highly ligand efficient and selective N-2-(Thioethyl)picolinamide histone deacetylase inhibitors inspired by the natural product psammaplin A (2013) 10. Total synthesis and full histone deacetylase inhibitory profiling of Azumamides A-E as well as β²- epi-Azumamide E and β³-epi-Azumamide E (2013)
• Description: Human HDAC8 , GenBank Accession No. NM_018486, full length with C-terminal His-tag, MW= 46.4 kDa, expressed in a baculovirus expression system.
• Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 225 mM imidazole, and 10% glycerol.
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
• Host Species: insect
• Product Type: Protein
• Host Cell Line: Sf9 cells
• Biosafety Level: Not applicable (BSL-1)
• Unit Definition: One U =1 pmol of acetyl group removed/min/µg of enzyme
• Assay Conditions: 25 mM Tris HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 µM BPS HDAC class 2a substrate (Catalog #50040), and HDAC8 (3.1 - 200 ng). Incubation condition: 30 min at 37°C, followed by_x000D_ HDAC developer (Catalog #50030) for 15 min at room temperature. Fluorescence intensity is measured at ex360/em460.
• Related Products: 50068, 50069, 50009, 50040
• Specific Activity: ≥300 pmol/min/µg.
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Deacetylase