FBLIM1 (NM_001024215) Human Tagged ORF Clone

DESCRIPTION:

FBLIM1 (Myc-DDK-tagged)-Human filamin binding LIM protein 1 (FBLIM1), transcript variant 2

DETAILS

Mw: 40.1 kDa
Tag: Myc-DDK
Accn: NM_001024215
Type: Human Tagged ORF Clone
Refseq: NM_001024215.1, NP_001019386.1
Status: In Stock*
Symbol: FBLIM1
Vector: pCMV6-Entry
Category: cDNA Clones
Locus Id: 54751
Orf Size: 1122 bp
Synonyms: CAL; FBLP-1; FBLP1
Refseq Orf: 1125 bp
Uniprot Id: Q8WUP2
Refseq Size: 1521 bp
Availability: In Stock
Cytogenetics: 1p36.21
Gene Summary: This gene encodes a protein with an N-terminal filamin-binding domain, a central proline-rich domain, and, multiple C-terminal LIM domains. This protein localizes at cell junctions and may link cell adhesion structures to the actin cytoskeleton. This protein may be involved in the assembly and stabilization of actin-filaments and likely plays a role in modulating cell adhesion, cell morphology and cell motility. This protein also localizes to the nucleus and may affect cardiomyocyte differentiation after binding with the CSX/NKX2-5 transcription factor. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Chromatograms: CHROMATOGRAMS Sequencher program is needed, download here.
Sequence Data: ORF Nucleotide Sequence ( show hide ) >RC224749 representing NM_001024215 Red =Cloning site Blue=ORF Green =Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGC C ATGGCCTCAAAGCCTGAGAAGAGGGTGGCATCGTCTGTCTTTATCACCCTGGCACCCCCGCGCCGCGATG TGGCCGTGGCGGAGGAAGTGAGGCAGGCAGTTTGTGAGGCCCGGCGTGGCCGCCCCTGGGAGGCTCCTGC CCCCATGAAGACACCCGAGGCTGGCTTGGCGGGGAGGCCCAGCCCCTGGACAACCCCTGGCAGAGCTGCA GCCACAGTGCCGGCTGCACCTATGCAGCTCTTCAATGGAGGATGCCCACCCCCTCCTCCTGTCCTGGATG GTGAGGACGTGCTTCCTGACCTGGACCTCCTCCCACCCCCTCCACCGCCCCCTCCAGTGCTTCTGCCTTC TGAAGAGGAGGCTCCTGCTCCAATGGGGGCCTCACTCATTGCAGACTTAGAGCAGCTGCACCTGTCCCCG CCCCCGCCCCCACCACAGGCCCCAGCGGAGGGACCTTCAGTCCAGCCCGGTCCCCTCAGGCCCATGGAGG AAGAGCTGCCACCTCCCCCGGCAGAACCTGTTGAGAAAGGGGCATCCACAGACATCTGTGCCTTCTGCCA CAAGACCGTGTCCCCCCGAGAGCTGGCTGTGGAGGCCATGAAGAGGCAGTACCATGCCCAGTGCTTCACG TGCCGCACCTGCCGCCGCCAGCTGGCTGGGCAGAGCTTCTACCAGAAGGATGGGCGACCCCTCTGCGAAC CCTGCTACCAGGACACACTGGAGAGGTGCGGCAAGTGTGGCGAGGTGGTCCGGGACCACATCATCAGGGC CCTGGGCCAGGCCTTCCACCCCTCCTGCTTCACGTGTGTGACCTGCGCCCGGTGCATTGGGGATGAGAGC TTTGCCCTGGGCAGCCAGAACGAGGTGTACTGCCTGGACGACTTCTACAGGTACGAGAAGGGTTTGTGCA CTGGGTGGGGTGCAGGGACAGGGCGAGACCCAAGCAGGGTGAAGGAGCTGAGCTTGAGTCCTGGGTGCTG GGCCAGAGTTTCCTGTCTACTGGTTTATTATAAAGAATATTACAGGGCTGGGCTGGGCGCGGTGGCTCAC GCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGTGGATCACGAGGTCAGGAGATCGAGATCATCCTG GC ACGCGT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAG GTTTAA Protein Sequence ( show hide ) >RC224749 representing NM_001024215 Red =Cloning site Green =Tags(s) MASKPEKRVASSVFITLAPPRRDVAVAEEVRQAVCEARRGRPWEAPAPMKTPEAGLAGRPSPWTTPGRAA ATVPAAPMQLFNGGCPPPPPVLDGEDVLPDLDLLPPPPPPPPVLLPSEEEAPAPMGASLIADLEQLHLSP PPPPPQAPAEGPSVQPGPLRPMEEELPPPPAEPVEKGASTDICAFCHKTVSPRELAVEAMKRQYHAQCFT CRTCRRQLAGQSFYQKDGRPLCEPCYQDTLERCGKCGEVVRDHIIRALGQAFHPSCFTCVTCARCIGDES FALGSQNEVYCLDDFYRYEKGLCTGWGAGTGRDPSRVKELSLSPGCWARVSCLLVYYKEYYRAGLGAVAH ACNPSTLGGRGGWITRSGDRDHPG TR TRPLEQKLISEEDLAANDILDYKDDDDK V
Oti Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Oti Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
E Coli Selection: Kanamycin (25 ug/mL)
Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Mammalian Cell Selection: Neomycin