SIGLEC7 (D-siglec) Antibody (C-term) Blocking peptide

DETAILS

Format: Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed.
Gene Id: 27036
Category: Peptides; Blocking Peptides
Subtitle: Synthetic peptide
Gene Name: SIGLEC7
Other Names: Sialic acid-binding Ig-like lectin 7, Siglec-7, Adhesion inhibitory receptor molecule 1, AIRM-1, CDw328, D-siglec, QA79 membrane protein, p75, CD328, SIGLEC7, AIRM1
Availability: 2 weeks
Bio Background: SIGLECs are cell surface proteins of the Ig superfamily. Most SIGLECs have 1 or more cytoplasmic immune receptor tyrosine-based inhibitory motifs, or ITIMs. A large subgroup of SIGLECs share high homology with SIGLEC3 (CD33) and are localized to 19q13.4. The cDNA for the SLG gene encodes 2 variants, SLG-long (SLGL) and SLG-short (SLGS). The 595-amino acid SLGL protein contains a signal peptide and 2 V-set N-terminal Ig-like domains. The 477-amino acid SLGS protein has a weak signal sequence and, like most SIGLEC3-like SIGLECs, has only 1 V-set N-terminal Ig-like domain. Both variants contain 2 C2-set N-terminal Ig-like domains, a transmembrane domain, and a cytoplasmic tail with a putative ITIM and a putative SLAM-like tyrosine-based motif. The conserved arginine residue thought to be essential for sialic acid binding in other SIGLECs is replaced by a glutamine in SLGS and by a cysteine in SLGL. RT-PCR analysis detected high expression of both variants in spleen and small intestine, and SLGS was highly expressed in adrenal gland and SLGL was highly expressed in bone marrow.
Bio References: Nicoll, G., et al., Eur. J. Immunol. 33(6):1642-1648 (2003).Alphey, M.S., et al., J. Biol. Chem. 278(5):3372-3377 (2003).Angata, T., et al., Glycobiology 10(4):431-438 (2000).Falco, M., et al., J. Exp. Med. 190(6):793-802 (1999).Nicoll, G., et al., J. Biol. Chem. 274(48):34089-34095 (1999).
Other Accession: Q9NZQ1
Primary Accession: Q9Y286
Targetspecificity: The synthetic peptide sequence used to generate the antibody AP1633b was selected from the C-term region of human D-siglec . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.