DESCRIPTION

• Tags: AMC
• Format: DMSO solution
• Shiptemp: -20°C or -80°C
• Warnings: Protect from direct exposure to light. Avoid freeze/thaw cycles.
• Category: Deacetylase/Substrates
• References: 1. A. Ito et al. (2001) EMBO J. 20 1331. 2. A. Ito et al. (2002) EMBO J. 21 6236.3. N.A. Barlev et al. (2001) Mol. Cell 8 1243.
• Description: A fluorogenic, acetylated peptide substrate for HDAC2, HDAC3, and HDAC6 (tested so far). This substrate fluoresces at excitation 350-380 nm, emission at 440-460 nm. It should be used in conjunction with HDAC Developer, Cat. # 50030.
• Supplied As: 5 mM DMSO solution (100 µl)
• Unspsc Code: 41105331
• Unspsc Name: Protein chemifluorescent detection reagents or kits or substrates
• Applications: Study enzyme kinetics, and screen small molecule HDAC inhibitors for drug discovery and HTS applications.
• Product Type: Substrate
• Biosafety Level: Not applicable (BSL-1)
• Storage Stability: Stable at least 12 months from date of receipt, when stored as directed
• Scientific Category: Deacetylase
• Instructions for Use: Materials : HDAC Assay buffer (BPS catalog #50031) HDAC Assay developer (BPS catalog #50030) Fluorogenic HDAC Substrate (BPS catalog #50032)HDAC2 (0.1 µg/µl) (BPS catalog #50001) or other class 1 or class 2b HDAC enzyme Step 1 : Add the following reagents to a low binding NUNC black plate (VWR catalog #62408-936): HDAC Assay Buffer, 39 µl1 mg/ml BSA, 5 µl 200 µM HDAC substrate, 5 µlHDAC2, 1 µl Incubate at 37°C for 30 min. Step 2 : Stop the reaction by adding 50 µl of HDAC assay developer (undiluted) and incubate the plate at room temperature for 15 min. Step 3 : Read samples in a microtiter-plate reading fluorimeter capable of excitation at 350-380 nm and emission at 440-460 nm. "Blank" value (no enzyme negative control) is subtracted from all other values.