DESCRIPTION

• Aa: 321-609
• Mw: 32.6 kDa
• Tags: His-tag
• Format: Aqueous buffer solution
• Genbank: NM_203500
• Species: Human
• Uniprot: Q14145
• Shiptemp: -80°C (dry ice)
• Synonyms: Kelch-like ECH-associated protein, INRF2, KIAA0132, KLHL19, Nrf
• Warnings: Avoid freeze/thaw cycles. Storing diluted protein is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).
• Category: Ubiquitination/Ligases (E3)
• References: 1. Dinkova-Kostova, A.T., et al., 2002. Proc. Nat. Acad. Sci. (USA). 99(18): 11908-11913. 2. Inoyama, D., et al. 2012. J. Biomol. Screening 17(4): 435-447.
• Description: Human Keap1, also known as Kelch-like ECH-associated protein, INRF2, KIAA0132, and KLHL19, GenBank Accession No. NM_203500, a.a. 321-609 expressed in an E. coli cell expression system. MW = 32.6 kDa
• Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, 3 mM DTT, 20% glycerol
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for studying protein binding and screening small molecular inhibitors
• Product Type: Protein
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: Binding of Keap1 to Nrf2 was measured using BPS Keap1:Nrf2 Assay Kit, Cat. #72020. Briefly, varying concentrations of Keap1 were added to wells containing FITC-labeled Nrf2 peptide in assay buffer and incubated for 30 min. at room temp. Fluorescence was measured at _x0002_ex 485nm, _x0002_em 530 nm using a Bio-Tek fluorescent microplate reader.
• Related Products: 60513, 60514, 72020
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Oxidative Stress