DESCRIPTION

• Tag: Tag Free
• Accn: NM_146424
• Type: Mouse Untagged Clone
• Refseq: NM_146424.1, NP_666635.1
• Status: 4 Weeks*
• Symbol: Olfr888
• Vector: pCMV6-Entry
• Category: cDNA Clones
• Locus Id: 258416
• Synonyms: MOR162-4
• Refseq Orf: 945 bp
• Insert Size: 945 bp
• Refseq Size: 945 bp
• Availability: 4 Weeks
• Cytogenetics: 9 A4
• Gene Summary: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]
• Sequence Data: Fully Sequenced ORF ( show hide ) >MC213597 representing NM_146424 Red =Cloning site Blue =ORF Orange =Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGC C ATGATGTTGGGAAGAATGGCCTTCAGCAATGACTCTTCTGTGAAGGAGTTTATCCTGCTGGGCTTAACAC AGCAGCCAGAGCTCCAGATGCCTCTCTTCTTCCTCTTCTTGGGAATCTATGTGGTCTCCATGGTGGGGAA CCTGGGACTGATTGTTCTGATTGTGTTGAATCCTCACCTGCACACCCCCATGTACTACTTTCTCTTCAAC CTTTCCTTTATTGATCTCTGCTACTCTTCTGTCATAACCCCCAGAATGTTGGTGGGTTTTGTGAAGCAGA ACATCATCTCTCATGCAGAGTGTATGACTCAGCTCTTTTTCTTCTGCTTCTTTGTTATTGATGAATGCTA CATTTTGACAGCAATGGCCTATGACAGATATGCTGCCATCTGTAAGCCCCTGCTTTACCAGGTTACCATG TCTCATCAGGTCTGCCACTTGATGATGGTGGGAGTGTATGTGATGGGGCTTGTGGGTGCCATGGCACATA CTGGTAGCATGCTAAGTCTGACCTTCTGTGATGGCAACATTATCAATCACTACATGTGTGACATACCTCC TCTCCAGAAGCTCTCCTGCACAAGCACCTCCATCAATGAGCTGGTAGTTTTCATTGTTGTGGGTGTCAAT GTAATAATACCCAGTCTGACTGTTTTTATTTCTTACACGTTGATCCTTTCTAACATCCTCAGCATTCAAT CTGCAGAGGGTAGGTCGAAAGCCTTCAGTACGTGTGGCTCCCATGTGATTGCTGTTTCTCTTTTCTTTGG AGCTTCAGCATTCATGTACCTTAAGCCTTCTAGTGCATCTGTGGATGATGATAAAATATCTACCATATTT TATACCATTGTGGGCCCAATGTTGAATCCTTTCATTTACAGTTTAAGGAATAAGGATGTCCACATTGCAC TGAGAAAAACTTTGAAGAAAAGTATGTTTATCTAA ACGCGT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
• Oti Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• E Coli Selection: Kanamycin (25 ug/mL)
• Restriction Sites: SgfI-MluI     
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Mammalian Cell Selection: Neomycin