DESCRIPTION

• Tag: Tag Free
• Accn: NM_175750
• Type: Mouse Untagged Clone
• Refseq: NM_175750.3, NP_786926.2
• Status: 7 Weeks*
• Symbol: Plxna4
• Vector: pCMV6-Entry
• Category: cDNA Clones
• Locus Id: 243743
• Synonyms: 9330117B14; mKIAA1550; Plxa4
• Refseq Orf: 5682 bp
• Uniprot Id: Q80UG2
• Insert Size: 5682 bp
• Refseq Size: 12602 bp
• Availability: 7 Weeks
• Cytogenetics: 6 A3.3
• Gene Summary: Coreceptor for SEMA3A. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance in the developing nervous system. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm.[UniProtKB/Swiss-Prot Function]
• Sequence Data: Fully Sequenced ORF ( show hide ) >MC225017 representing NM_175750 Red =Cloning site Blue =ORF Orange =Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGC C ATGAAAGCCATGCCCTGGAACTGGACTTGCCTGCTCTCCCACCTGCTGGTGGTAGGGATGGGCTCCTCCA CTCTGCTTCCACGACAGCCACCCCAGCTGTCTCAGAAGCCTTCCTTTGTGACATTCCGAGGGGAGCCTGC TGAGGGCTTCAATCACCTTGTGGTGGATGAGAGGACAGGGCACATTTATTTGGGGGCTGTCAACAGAATC TACAAACTCTCTAGTGACCTCAAAGTCTTGGTGACTCATCAGACAGGGCCAGATGAGGACAACCCCAAGT GTTACCCACCTCGGATTGTTCAGACCTGCAATGAGCCTCTGGCCAGCACCAATAACGTCAACAAGATGCT GCTTATAGACTACAAAGAAAACAGGCTGATCGCGTGTGGGAGCCTGTACCAGGGTATCTGCAAGCTCCTG AGGCTAGAGGACCTCTTCAAGTTGGGAGAGCCCTTTCACAAGAAGGAACATTATCTCTCGGGGGTTAACG AGAGTGGCTCAGTCTTTGGGGTGATTGTTTCCTACAGCAACTTTGATGATAAACTCTTTATTGCCACAGC AGTGGATGGCAAACCTGAATATTTTCCTACCATCTCCAGCAGGAAGCTGACTAAGAACTCTGAGGCCGAT GGCATGTTTGCTTACGTTTTCCATGATGAATTTGTGGCCTCTATGATTAAGATCCCTTCAGACACTTTCA CGGTTATCCCCGACTTTGACATCTACTACGTCTATGGCTTCAGCAGTGGCAATTTTGTCTACTTTTTGAC CCTTCAGCCAGAGATGGTGTCTCCCCCAGGCTCCACGACAAAGGAGCAGGTGTACACCTCAAAGCTTGTG AGGCTTTGCAAAGAGGACACAGCCTTTAACTCCTACGTAGAGGTGCCCATTGGCTGTGAGCGCAATGGGG TGGAGTATCGCTTGTTACAGGCTGCCTACTTGTCTAAAGCTGGTGCAGTGCTTGGCCGGACCCTAGGAGT CCGCCCAGATGACGACCTCCTCTTCACCGTCTTCTCCAAGGGCCAGAAGCGGAAGATGAAATCTCTGGAT GAGTCTGCCCTGTGCATCTTCATCTTGAAGCAGATCAATGATCGCATTAAGGACCGCCTGCAGTCCTGTT ATAGGGGTGAGGGAACACTAGACCTGGCCTGGCTCAAGGTGAAGGACATCCCTTGCAGCAGTGCGCTCCT TACTATTGATGACAACTTCTGTGGCCTGGATATGAATGCTCCTCTGGGAGTTTCCGAAATGGTGCGTGGC ATTCCAGTCTTCACAGAAGACAGGGACCGCATGACTTCTGTCATCGCCTATGTCTACAAGAACCACTCAC TGGCCTTCGTGGGCACCAAAAGTGGCAAGCTGAAGAAGATCCGGGTTGATGGGCCCAAGGGAAATGCCCT CCAATATGAGACTGTGCAGGTGGTGGACTCAGGACCAGTCCTCCGGGACATGGCCTTTTCCAAGGACCAC GAGCAACTCTACATCATGTCAGAAAGGCAGCTTACCAGAGTTCCTGTGGAGTCCTGTGGGCAGTACCGGA GCTGTGGCGAGTGTCTTGGCTCAGGTGACCCCCATTGTGGCTGGTGTGTGCTCCACAACACGTGCACCCG GAAGGAACGGTGTGAACGCTCCAGAGAACCCCGAAGGTTTGCCTCAGAGATGAAGCAGTGTGTCCGGCTG ACGGTCCATCCCAACAACATCTCTGTCTCTCAGTACAACGTGCTGCTGGTCCTAGAGACATACAATGTCC CGGAGCTGTCAGCAGGTGTCAACTGTACCTTTGAAGATCTGTCAGAGATGGATGGGCTGGTAATAGGCAA TCAGATCCAGTGCTACTCCCCTGCAGCCAAGGAAGTGCCCCGGATCATCACAGAAAATGGGGACCATCAT GTTGTCCAGCTGCAACTCAAGTCAAAGGAGACTGGGATGACCTTCGCCAGCACCAGCTTTGTCTTCTACA ACTGCAGTGTCCACAATTCATGCCTGTCCTGTGTGGAGAGCCCGTACCGTTGCCACTGGTGTAAATACCG GCATGTTTGTACTCACGACCCCAACACATGCTCCTTCCAGGAAGGCCGAGTGAAGCTACCTGAGGACTGC CCCCAGCTGCTGCGAGTGGACAAGATCCTCGTTCCAGTAGAGGTGATCAAGCCCATCACTCTGAAGGCTA AGAACCTCCCACAGCCTCAGTCGGGACAGAGAGGCTACGAGTGCATCTTGAACATCCAGGGCATCGAGCA GAGGGTACCTGCTCTGCGCTTCAACAGCTCCAGTGTGCAGTGTCAGAACACCTCCTATTCCTATGAAGGA ATGGAGATCAACAACCTGCCAGTGGAGTTGACAGTCGTGTGGAATGGACACTTCAACATTGACAACCCAG CTCAGAATAAAGTTTACCTCTACAAGTGTGGAGCCATGCGCGAGAGCTGTGGGCTGTGCCTCAAGGCCGA CCCGGATTTCGAGTGTGGCTGGTGTCAGAGCCCAGGCCAGTGTACCCTGCGCCAGCACTGCCCTGCCCAT GAGAGCCGGTGGTTGGAACTGTCGGGGGCCAACAGCAAGTGCACCAACCCTCGTATCACAGAGATCATCC CAGTGACGGGCCCTCGAGAAGGGGGCACCAAGGTCACCATCCGAGGGGAGAACTTGGGCCTGGAATTCCG TGACATTGCATCACACGTGAAAGTGGCTGGTGTGGAATGCAGCCCCTTGGTGGATGGCTACATCCCAGCT GAACAGATCGTGTGTGAGATGGGTGAGGCCAAGCCTAGCCAGCATGCAGGCTTTGTGGAGATCTGTGTGG CTGTGTGTCGGCCTGAGTTCATGGCCCGCTCTTCGCAGCTCTATTACTTCATGACTCTGACCCTCGCAGA TCTGAAGCCCAACCGAGGACCCATGTCTGGGGGGACACAGGTTACCATCACAGGTACCAACTTGAATGCA GGCAGCAATGTTGTGGTGATGTTCGGGAGCCAGCCCTGCCTCTTCCACAGGCGCTCTCCATCCTACATTA TCTGTAACACTACATCCTCAGAGGAGGTGCTGGACATGAAGGTGACTGTGCAGGTGGACAGGGCCAGGAT CCGCCAGGACCTGGTCTTTCAGTACGTGGAGGACCCCACCATTGTGCGGATTGAACCAGAATGGAGCATT GTCAGTGGGAACACACCTATTGCTGTCTGGGGAACTCACCTGGACCTCATACAGAACCCACAGATCCGTG CCAAGCATGGAGGAAAAGAACACATCAACATCTGTGAGGTCCTAAATGCTACAGAGATGACCTGCCAGGC TCCAGCCCTTGCCCTGGGTCCCGACCACCAGTCAGATCTCACTGAGAGGCCTGAAGAATTTGGTTTCATC CTGGACAATGTCCAGTCTCTGCTAATTCTCAACAAGACCAACTTCACCTATTATCCTAACCCCGTGTTTG AGGCTTTCAGTCCCTCGGGAATCCTGGAGCTCAAGCCGGGCACCCCCATTATCCTAAAGGGCAAGAACTT GATCCCGCCTGTGGCCGGAGGCAATGTGAAGCTGAACTACACCGTGCTGGTTGGGGAGAAACCATGCACC GTGACAGTATCAGATGTGCAGCTGCTCTGCGAGTCTCCCAACCTCATCGGCAGGCACAAAGTGATGGCAC GGGTGGGTGGCATGGAGTATTCCCCTGGGATGGTATACATCGCCCCAGACAGCCCGCTCAGCCTGCCCGC CATTGTCAGCATCGCGGTGGCTGGTGGCCTCCTCATCATCTTCATCGTGGCCGTACTCATCGCCTACAAA CGCAAATCCCGAGAAAGTGACCTCACGCTGAAGAGGTTGCAGATGCAGATGGACAACCTTGAGTCCCGTG TGGCGCTGGAGTGCAAGGAAGCTTTTGCCGAGCTGCAGACAGATATCCATGAGCTTACCAGTGACCTGGA TGGAGCTGGGATTCCCTTTCTGGACTACAGAACCTATACCATGCGGGTGCTGTTCCCAGGGATTGAAGAC CACCCTGTGCTACGAGACCTTGAGGTCCCAGGCTACCGGCAGGAGCGTGTGGAGAAAGGACTCAAGCTCT TCGCCCAGCTCATCAACAACAAGGTGTTCCTGCTCTCTTTCATCCGCACTCTGGAGTCTCAGCGCAGTTT TTCCATGCGCGACCGAGGTAACGTGGCCTCCCTCATCATGACGGTACTTCAGAGCAAGCTAGAGTATGCC ACTGACGTGCTGAAGCAGCTACTGGCTGACCTCATTGATAAGAACCTGGAGAGCAAGAACCACCCCAAGC TGCTGCTCAGGAGGACGGAGTCGGTGGCCGAGAAGATGTTGACTAATTGGTTCACTTTCCTCCTCTACAA GTTCCTCAAGGAGTGTGCTGGGGAGCCACTCTTCTCCCTGTTCTGTGCCATCAAGCAGCAGATGGAGAAG GGCCCCATTGATGCCATCACCGGAGAGGCCCGCTACTCGCTAAGTGAGGACAAACTCATCCGACAGCAGA TTGAATATAAAACCTTGGTCCTGAGCTGTGTCAGTCCAGATAATGTTAACAGCCCAGAGGTCCCAGTGAA GATCCTCAACTGTGACACCATCACTCAGGTCAAAGAGAAGATCCTAGATGCCATCTTCAAGAATGTCCCG TGCTCCCATAGGCCCAAAGCTGCAGACATGGATCTAGAGTGGCGACAAGGAAGTGGGGCGAGGATGATCC TGCAGGACGAAGATATCACCACCAAGATCGAGAATGATTGGAAGAGACTCAACACAGTAGCCCACTACCA GGTACCAGATGGTTCTGTGGTGGCTTTAGTGTCCAAGCAAGTGACAGCCTACAATGCAGTGAACAACTCC ACTGTCTCCAGGACTTCAGCAAGTAAATATGAGAACATGATCCGGTACACAGGCAGCCCTGACAGCCTCC GTTCCCGGACACCCATGATCACCCCTGACCTGGAAAGCGGGGTCAAGCTTTGGCATCTGGTGAAGAACCA TGAACATGGAGACCAGAAGGAGGGGGACCGGGGGAGCAAGATGGTGTCTGAGATCTATCTGACACGACTA TTGGCCACAAAGGGCACACTGCAGAAGTTTGTGGATGACCTATTCGAGACCATCTTCAGTACGGCCCACC GTGGCTCCGCTCTGCCGCTAGCCATCAAGTACATGTTTGACTTCCTCGATGAGCAGGCTGATAAGCACGG CATCCATGACCCGCACGTCCGCCACACCTGGAAGAGCAACTGCCTGCCCCTACGGTTTTGGGTGAATATG ATCAAGAACCCTCAGTTTGTGTTTGACATCCATAAGAACAGCATCACGGATGCCTGTCTCTCTGTGGTGG CCCAGACCTTCATGGACTCCTGCTCCACTTCGGAGCACCGACTGGGCAAGGATTCCCCCTCCAACAAGCT GCTCTATGCCAAGGACATCCCCAGCTACAAGAACTGGGTAGAGAGGTATTATTCAGACATTGGGAAGATG CCGGCAATAAGTGACCAGGACATGAATGCATACCTGGCCGAGCAGTCGCGGATGCACATGAATGAGTTCA ATACGATGAGTGCGCTCTCAGAGATCTTCTCCTATGTGGGCAAATACAGTGAGGAGATCCTTGGACCCCT GGACCATGATGACCAGTGTGGAAAGCAGAAACTGGCTTACAAACTAGAACAAGTCATAACTCTCATGAGC TTAGACAGCTGA ACGCGT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
• Oti Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• E Coli Selection: Kanamycin (25 ug/mL)
• Restriction Sites: SgfI-MluI     
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Mammalian Cell Selection: Neomycin