DESCRIPTION

• Tag: C-His
• Purity: ≥ 92 % as determined by SDS-PAGE. ≥ 85 % as determined by SEC-HPLC.
• Source: HEK293 Cells
• Species: Human
• Storage: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Activity: 1. Immobilized human TNFRI-His at 10 μg/mL (100 μl/well) can bind biotinylated human TNFα. The EC50 of biotinylated human TNFα is 5-12 μg/mL. 2. Measured by its ability to inhibit TNFα-mediated cytotoxicity in the L929 mouse fibrosarcoma cells in the presence of metabolic inhibitor actinomycin D. The ED50 for this effect is typically 0.05-0.4μg/mL in the presence of 0.25 ng/mL recombinant human TNFα.
• Molecule: TNFRSF1A
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Lead Time: 1-3 days
• Formulation: Lyophilized from sterile PBS, pH 7.4
• Molecular Mass: The recombinant human TNFRI consists of 201 amino acids after removal of the signal peptide and has a predicted molecular mass of 22.7 kDa. As a result of glycosylation, the apparent molecular mass of rh TNFRI is 30-35 kDa in SDS-PAGE under reducing conditions.
• Reconstitution: A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
• Accession Number: NP_001056.1
• Predicted N Terminal: Ile 22