Biotinylated Mouse PSMA / FOLH1 Protein, His,Avitag™

DESCRIPTION

Prostate-specific membrane antigen (PSMA) is also known as Folate hydrolase 1 (FOLH1), Glutamate carboxypeptidase 2 (GCP2), N-acetylated-alpha-linked acidic dipeptidase I (ALAD1), which belongs to the peptidase M28 family and M28B subfamily. FOLH1 / PSMA is stable at pH greater than 6.5. FOLH1 / PSMA is a type II transmembrane zinc metallopeptidase that is most highly expressed in the nervous system, prostate, kidney, and small intestine. FOLH1 / GCP-2 is homodimer and binds 2 zinc ions per subunit, and required for ALADase activity. The catalytic activity of PSMA involved in releasing of an unsubstituted, C-terminal glutamyl residue, typically from Ac-Asp-Glu or folylpoly – gamma - glutamates. FOLH1 / GCP-2 / PSMA has both folate hydrolase and N – acetylated – alpha – linked - acidic dipeptidase (ALADase) activity and has a preference for tri-alpha-glutamate peptides. GCP-2 / PSMA involved in prostate tumor progression and also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.

DETAILS

Tag: N-10×His & N-Avi
Host: HEK293
Size: 25ug
Buffer: 25 mM MES, 500 mM NaCl, 1 mM CaCl2, 1 mM MgCl2, 0.5 mM ZnCl2, pH6.5
Format: Liquid
Purity: 95%
Source: Biotinylated Mouse PSMA Protein, His,Avitag (PSA-M82Q4) is expressed from human 293 cells (HEK293). It contains AA Ile 44 - Ala 752 (Accession # O35409-1).
Storage: -70℃
Category: MABSol® Biotin Labeled Proteins
Molecule: PSMA
Conjugate: Biotin-labeled
Stability: ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Exp Region: Ile 44 - Ala 752
Swiss-prot: O35409-1
Package Size: 25ug*1
Characteristics: This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 83.2 kDa. The protein migrates as 95-120 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin Level: 1.0 EU per μg
Molecular Weight: 83.2 kDa
Shipping Condition: Dry ice
Species Reactivity: Mouse
Extra Shippinghandling Fee: Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/A48-Shipping-and-Payments.html