Anti-PKCθ (N-terminal region) Antibody

DETAILS

Form: Protein A Purified
Gene: PRKCQ
Host: Mouse
Rrid: AB_2172086
Type: Primary Antibody
Clone: M217
Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Target: PKCθ (N-terminal region)
Isotype: IgG2a
Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Shipping: Blue Ice
Synonyms: PKC theta
Clonality: Mouse Monoclonal
Immunogen: Clone (M217) was generated from a recombinant mouse PKCθ protein that included amino acids residues in the N-terminal region. This sequence is conserved in human and rat PKCθ, and has low homology to other PKC family members.
Expiration: After date of receipt, stable for at least 1 year at -20°C.
Specificity: This antibody detects a 79 kDa* protein corresponding to the molecular mass of PKCθ on SDS-PAGE immunoblots of human Jurkat cell lysates.
Applications: WB, ICC
Conjugate Exem: Unconjugated
Physical State: Liquid
Quality Control: Western blots performed on each lot.
Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Molecular Weight: 79
Dilution Range Wb: 1:1000
Immunogen Species: Mouse
Dilution Range Icc: 1:100
Species Reactivity: Human, Mouse, and Rat
Target Description: The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half interacts with PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester, while the C-terminal half contains the catalytic domain. The conventional PKC subfamily (α, β1, βII, and γ) is regulated by both Ca2+ and diacylglycerol. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters, and growth factors. The phosphorylation of multiple sites in PKCs regulates their activity
Uniprot Number Immunogen Species: A6H667
Post Translational Modification Type: GOmAb