CM3541
Anti-δ1-Catenin (central region) Antibody
PhosphoSolutions
DETAILS
- Form: Protein A Purified
- Gene: CTNND1
- Host: Mouse
- Rrid: AB_1769552
- Type: Primary Antibody
- Clone: M354
- Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
- Target: δ1-Catenin (central region)
- Isotype: IgG1
- Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
- Shipping: Blue Ice
- Synonyms: pp120 Src substrate, p120
- Clonality: Mouse Monoclonal
- Immunogen: Clone (M354) was generated from a peptide that includes amino acids from the central region of human δ1-Catenin. This peptide sequence is highly conserved in rat and mouse δ1-Catenin.
- Expiration: After date of receipt, stable for at least 1 year at -20°C.
- Specificity: The antibody detects a 110 kDa* protein corresponding to the molecular mass of δ1-Catenin on SDS-PAGE immunoblots of human A431 and HUVEC cells.
- Applications: WB, ICC, IP
- Conjugate Exem: Unconjugated
- Physical State: Liquid
- Quality Control: Western blots performed on each lot.
- Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
- Molecular Weight: 110
- Dilution Range Ip: 1:50
- Dilution Range Wb: 1:1000
- Immunogen Species: Human
- Dilution Range Icc: 1:100
- Species Reactivity: Human, Mouse, and Rat
- Target Description: Catenins have emerged as molecular sensors that integrate cell-cell junctions and cytoskeletal dynamics with signaling pathways that control morphogenesis and cell to cell communication. δ1-Catenin (p120 catenin) is a catenin family member which contains an N-terminal coiled-coil domain, a regulatory domain containing multiple phosphorylation sites, and a central Armadillo repeat domain. δ1-Catenin regulates E-cadherin turnover, and has both positive and negative effects on cadherin-mediated adhesion. Actin dynamics are also regulated by δ1-Catenin, which can modulate RhoA, Rac and cdc42 activity. δ1-Catenin is phosphorylated at multiple tyrosine, serine and threonine sites both in vitro and in vivo. High levels of δ1-Catenin phosphorylated at Tyr-228 are commonly seen in several carcinoma cell lines and after EGFR activation. Many other tyrosine sites are also phosphorylated in the N-terminal region including Tyr-96, Tyr-112, Tyr-280, and Tyr-302. In addition, Thr-310 and Thr-916 are constituitively phosphorylated in many cell types, however this phosphorylation may occur only in δ1-Catenin associated with the plasma membrane.
- Uniprot Number Immunogen Species: O60716
- Post Translational Modification Type: GOmAb