DESCRIPTION

• Notes: License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.
• Species: Human
• Shiptemp: -80°C (dry ice)
• Synonyms: Nuclear factor of activated T cells; T cell
• Warnings: Avoid freeze/thaw cycles.
• Category: Cell Signaling/Cell Line
• Background: The nuclear factor of activator T cells (NFAT) family of transcription factors plays an important role in immune response. T cell activation through the T cell synapse results in calcium influx. Increased intracellular calcium levels activate the calcium-sensitive phosphatase, calcineurin, which rapidly dephosphorylates the serine-rich region (SRR) and SP-repeats in the amino termini of NFAT proteins. This results in a conformational change that exposes a nuclear localization signal promoting NFAT nuclear import. In the nucleus, NFAT proteins cooperate with other proteins to bind to DNA.
• References: 1. Clipstone NA, Crabtree GR. Nature. 1992 Jun 25;357(6380):695-7. 2. Lyakh, L., et al. Mol Cell Biol. 1997 May;17(5):2475-84.
• Description: The NFAT Reporter - Jurkat Cell Line contains a firefly luciferase gene under the control of the NFAT response element stably integrated into Jurkat cells. This cell line has been validated for response to thapsigargin, ionomycin, and phorbol 12-myristate 13-acetate (PMA). It is useful as a control cell line for other NFAT reporter cell lines expressing various immune checkpoint receptors.
• Supplied As: Each vial contains ~2x106 cells in 1 ml of 10% DMSO.
• Unspsc Code: 41106514
• Unspsc Name: Stable mammalian cell lines
• Applications: • Monitor intracellular calcium levels. • Screen for activators or inhibitors of PKC/ Ca2+ pathway. • Screen for agonists or antagonists of T cell receptors. • Control for immune checkpoint NFAT reporter cell lines.
• Host Species: human
• Product Type: Cell Line
• Host Cell Line: Jurkat
• Biosafety Level: BSL-1
• Related Products: 79530, 60690, 60184, 100182, 79796
• Storage Stability: Immediately upon receipt, store vials in liquid nitrogen.
• Mycoplasma Testing: The cell line has been screened using the PCR-based Venor™GeM Mycoplasma Detection kit (Sigma-Aldrich) to confirm the absence of Mycoplasma species.
• Scientific Category: Immunotherapy
• Instructions for Use: See data sheet for detailed culturing and assay protocol.
• Required Accessories: 60690,79796,79530,60184
• Signed License Required: Yes