Spike Trimer (S1+S2) (B.1.351, Beta Variant, Δ242-244) (SARS-CoV-2) Recombinant

DESCRIPTION

Recombinant SARS-CoV-2 Spike protein in its homotrimeric form, containing S1+S2 subunits and encompassing amino acids 16-1213. This protein corresponds to SARS-CoV-2 Variant B.1.351, also known as variant Beta originally discovered in South Africa, and contains the nine known mutations listed below, in addition to deletion 242-244. It also contains mutations 682RRAR685>A, K986P and V987P. The construct has a C-terminal His-tag (6xHis). Note that the expected MW of the S1+S2 monomer is 137kDa but migrates at a higher MW in SDS-PAGE due to glycosylation. The recombinant protein was affinity purified.*Deletion 242-244 is known as 241-243 in some databases and on the CDC website. This is the same deletion. As stated by Tegally et al., Nature 2021: "We also observe a deletion of three amino acids at positions 242 to 244, which was seen in samples extracted and generated in different laboratories across the NGS-SA. This region is difficult to align; the deletion could potentially also be located at positions 241 to 243, but the resulting sequence would be exactly the same. "

DETAILS

Aa: 16-1213
Mw: 137 kDa + glycans
Tags: C-terminal His-tag
Format: Aqueous buffer solution
Purity: ≥90%
Genbank: MN908947
Species: SARS-CoV-2
Uniprot: P0DTC2
Mutation: L18F, D80A, D215G, R246I, K417N, E484K, N501Y, D614G, A701V, Δ242-244
Shiptemp: -80°C (dry ice)
Synonyms: coronavirus, covid, covid-19, covid19, Sars-cov-2 B1351, 20H/501Y.V2, sars cov 2 Spike Trimer, sarscov2, South African Variant, B1351 mutation, South Africa coronavirus, SARS-CoV-2 S. African, β, beta, deletion 242-244, deletion 241-243
Warnings: Avoid freeze/thaw cycles.
Category: Coronavirus/Protein
Constructs: Spike Trimer (S1+S2) (B.1.351 Variant, ˆ†242-244) (16-1213-His) (SARS-CoV-2)
Description: Recombinant SARS-CoV-2 Spike protein in its homotrimeric form, containing S1+S2 subunits and encompassing amino acids 16-1213. This protein corresponds to SARS-CoV-2 Variant B.1.351, also known as variant Beta originally discovered in South Africa, and contains the nine known mutations listed below, in addition to deletion 242-244. It also contains mutations 682RRAR685>A, K986P and V987P. The construct has a C-terminal His-tag (6xHis). Note that the expected MW of the S1+S2 monomer is 137kDa but migrates at a higher MW in SDS-PAGE due to glycosylation. The recombinant protein was affinity purified.*Deletion 242-244 is known as 241-243 in some databases and on the CDC website. This is the same deletion. As stated by Tegally et al., Nature 2021: "We also observe a deletion of three amino acids at positions 242 to 244, which was seen in samples extracted and generated in different laboratories across the NGS-SA. This region is difficult to align; the deletion could potentially also be located at positions 241 to 243, but the resulting sequence would be exactly the same. "
Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, 20% glycerol
Unspsc Code: 12352202
Unspsc Name: Proteins
Product Type: Protein
Biosafety Level: Not applicable (BSL-1)
Related Products: 100728, 510333, 100978, 78174
Storage Stability: At least 6 months at -80°C.
Scientific Category: Coronavirus