EM2331
Anti-ERK1 (C-terminal region) Antibody
PhosphoSolutions
DETAILS
- Form: Protein A Purified
- Gene: MAPK1
- Host: Mouse
- Rrid: AB_2139825
- Type: Primary Antibody
- Clone: M233
- Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
- Target: ERK1 (C-terminal region)
- Isotype: IgG1
- Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
- Shipping: Blue Ice
- Synonyms: ERK, p42, p44, MAPK
- Clonality: Mouse Monoclonal
- Immunogen: Clone (M233) was generated from a recombinant human ERK1 protein that included amino acids residues in the C-terminal region. This sequence is conserved in rat and mouse ERK1, and has low homology to ERK2, as well as other ERK family members.
- Expiration: After date of receipt, stable for at least 1 year at -20°C.
- Specificity: The antibody detects a 44 kDa* protein corresponding to ERK1 on SDS-PAGE immunoblots of human A431 epithelial cells. It does not detect ERK2.
- Applications: WB, ICC, IHC
- Conjugate Exem: Unconjugated
- Physical State: Liquid
- Quality Control: Western blots performed on each lot.
- Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
- Molecular Weight: 44
- Dilution Range Ip: 1:100
- Dilution Range Wb: 1:1000
- Immunogen Species: Human
- Dilution Range Icc: 1:100
- Species Reactivity: Human, Mouse, and Rat
- Target Description: Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The ERK1/2 (p44/42) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines. Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK), a MAP kinase kinase (MAPKK), and a MAP kinase (MAPK). Multiple ERK1/2 MAPKKKs have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway. MEK1 and MEK2 activate ERK1 and ERK2 through phosphorylation of activation loop residues Thr-202/Tyr-204 and Thr-185/Tyr-187, respectively. ERK1/2 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases. Several downstream targets of ERK1/2 have been identified, including p90RSK and the transcription factor Elk-1.
- Uniprot Number Immunogen Species: P28482
- Post Translational Modification Type: GOmAb