DESCRIPTION

• Aa: 339-459
• Mw: 15.1 kDa
• Tags: N-terminal His-tag
• Format: Aqueous buffer solution
• Genbank: NM_005104
• Species: Human
• Uniprot: P25440
• Shiptemp: -80°C (dry ice)
• Synonyms: BRD2, RING, RNF3, Bromodomain containing 2
• Warnings: Avoid freeze/thaw cycles.
• Category: Bromodomain/Protein
• References: 1. Umehara, T., et al., FEBS Lett. 2010 Sep 24; 584(18): 3901-3908.2. Shang, E., et al., J Cell Biochem. 2011 Oct; 112(10): 2784-2793.
• Description: Human Bromodomain containing 2, also known as BRD2 or RNF3, GenBank Accession No. NM_005104, a.a. 339 - 459 corresponding to bromdomain 2 with N-terminal His-tag, MW = 15.1 kDa, expressed in an E. coli expression system.
• Formulation: 50 mM Tris-HCl, pH 8.0, 138 mM NaCl, 2.7 mM KCl, 0.05% Tween-20, and 10% glycerol
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.
• Host Species: E. coli
• Product Type: Protein
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: BRD2 is incubated with biotinylated acetyl-histone peptide substrate (BPS #33000) in BRD assay buffer (BPS #33001) in a 10 µl reaction for 1 hour at RT. AlphaLISA® Nickel Chelate acceptor beads (PerkinElmer) are added, followed by Alphascreen® Streptavidin-conjugated donor beads (PerkinElmer). After 1 hour minute incubation at RT, Alphacounts are measured.
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Bromodomain
• Instructions for Use: Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.