FcGRIIB (CD32B) Lentivirus

DESCRIPTION

Fc Gamma Receptor 2B (FcGR2B, FcγRIIB), also known as CD32B, is a "low affinity" receptor for Immunoglobulin G (IgG). FcGR2B is involved in the phagocytosis of immune complexes and in the regulation of antibody production by B-cells. It is the predominant Fc receptor present on B cells. Crosslinking of antibodies by FcGR-expression cells can promote receptor clustering and increase downstream signaling. FcGR2B crosslinking is important for anti-TNFR receptor antibodies.The FcGRIIB Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types mammalian cells, including primary and non-dividing cells. The particles contain a FcGRIIB gene (NM_004001.4) driven by a CMV promoter.

DETAILS

Shiptemp: -80°C (dry ice)
Synonyms: CD32B Lentivirus
Warnings: Biosafety: None of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal. Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a Biosafety Level 2 facility. BPS recommends following all local federal, state, and institutional regulations and using all appropriate safety precautions.
Category: Cell Line/Lentiviruses
Description: Fc Gamma Receptor 2B (FcGR2B, FcγRIIB), also known as CD32B, is a "low affinity" receptor for Immunoglobulin G (IgG). FcGR2B is involved in the phagocytosis of immune complexes and in the regulation of antibody production by B-cells. It is the predominant Fc receptor present on B cells. Crosslinking of antibodies by FcGR-expression cells can promote receptor clustering and increase downstream signaling. FcGR2B crosslinking is important for anti-TNFR receptor antibodies.The FcGRIIB Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types mammalian cells, including primary and non-dividing cells. The particles contain a FcGRIIB gene (NM_004001.4) driven by a CMV promoter.
Formulation: The lentiviruses were produced from HEK293T cells in the medium containing 90% DMEM + 10% FBS.
Supplied As: Two vials (500 µl x 2) of lentivirus at a titer ≥5 x 106 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
Unspsc Code: 41106621
Unspsc Name: Virus mediated expression vectors or kits
Applications: 1. Transient expression of FcGRIIB in target cells.2. Generation of stable cell line expressing FcGRIIB with Geneticin (G418) selection.
Product Type: Lentivirus
Biosafety Level: BSL-2
Related Products: 79539, 79564, 79876, 79878
Storage Stability: Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the virus at -80°C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.
Scientific Category: Cell Signaling Pathway