302131-25 302131-100
Calcium Free Apoptotic-Dead Cell Tag - 488
BioMagis
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DESCRIPTION
Calcium Free Apoptotic-Dead Cell Tag – 488 is a probe specifically designed to bind to phosphatidylserine (PS) exposed on the outer leaflet of the cell membrane in apoptotic cells and dead cells, regardless of calcium levels. This probe is suitable for detecting apoptotic cells, dead cells, activated platelets, and extracellular vesicles using flow cytometry or fluorescence microscopy. Moreover, it can be used in multicolor staining alongside conjugated antibodies or dead cell indicators in calcium-free buffers. Importantly, the staining performed with the calcium-free apoptotic-dead cell tag is fixable, enabling the distinction of viable cells for subsequent intracellular staining.
DETAILS
- Application: FC, ICC
- Fluorophore: A488
- Formulation: Lyophilized
- Application Notes: Protocol for flow cytometer 1. Add 5 µL of the diluted reagent to 1 million cells in 100 µL of any staining buffer. No cell wash needed. 2. Incubate at room temperature for 5 minutes in the dark. 3. No wash needed. Directly running on the cytometer. Note: Different cell types can have a wide degree of variability in staining based on cell size and degree of cell death. Titrate the amount of dye and/or number of cells per 100 µl for optimal performance. Note: Cells can be fixed after staining.
- Excitationemission: Ex 488 / Em 520 nm
- Verified Reactivity: Human, Mouse
- Storage and Handling: • Store at -20°C upon receipt. Do not open vials until needed. • DMSO reconstitute solution of Apoptotic-Dead Cell Tag-dye can be stored at -20°C for up to six months. • Once diluted to dilution buffer can be stored at 2-8°C for up to three months.
- Reconstitution and Usage: • For 302131-100 / 100 Tests Reconstitute with 11 µl of DMSO, mix well, follow by another quick spin. Take the appropriate volume of DMSO solution (pre-calculate the volume as desired tests plus died volume), 50x dilute to dilution buffer (1 µL to 50 µL dilution buffer). Recommend 5 µl per 100 µl of any staining buffer for 1 million cells. • For 302131-25 / 25 Tests Reconstitute with 130 µL of dilution buffer. Mix well, follow by another quick spin. Let it site for 15 mins before using. Recommend 5 µl per 100 µl of any staining buffer for 1 million cells.