DESCRIPTION

• Mw: 88.9 kDa
• Tag: Myc-DDK
• Accn: NM_001302448
• Type: Mouse Tagged ORF Clone
• Refseq: NM_001302448.1, NP_001289377.1
• Status: 3 Weeks*
• Symbol: Rnf10
• Vector: pCMV6-Entry
• Category: cDNA Clones
• Locus Id: 50849
• Orf Size: 2415 bp
• Synonyms: AA675014; mKIAA0262; RIE2
• Refseq Orf: 2418 bp
• Uniprot Id: Q3UIW5
• Refseq Size: 3126 bp
• Availability: 3 Weeks
• Cytogenetics: 5 56.01 cM
• Gene Summary: The protein encoding this gene is a member of the really interesting new gene finger protein family. Members of this family contain protein motifs similar to zinc finger domains and are involved in many processes that include transcriptional regulation, DNA repair and signal transduction. Expression of this gene is upregulated during neuronal differentiation of cultured cells, and inhibition of its expression impairs differentiation and cell cycle exit, providing evidence for a function in neuronal differentiation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]
• Sequence Data: ORF Nucleotide Sequence ( show hide ) >MR231185 representing NM_001302448 Red =Cloning site Blue=ORF Green =Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGC C ATGCCGCAGAGCTCCCCCAGCGCCGCCGCCACCGCCTCCGACATGGACAAGAACAGCGGCTCCAACAGCT CCTCCGCCTCCTCGGGCAGCAGCAAAGGGCAACAGCCGCCCCGCTCCGCCTCGGCGGGGCCGGCCGGCGA GTCTAAACCCAAGAGTGATGGAAAGAACTCAAATGGATCCAAGCGTTACAATCGCAAGCGTGAACCCTCC TACCCCAAAAATGAAAATTTTAGCAACCAGTCCCGTCGCTCCAATTCACAGAAAAGCAAAACTTTTAACA AGATGCCTCCTCAAAGGGGCGGTGGCAGCAGCAAACCCTTTAGCTCTTCTTCTAATGGTGGAAGACGAGA TGAGGTAGCAGAGGCTCAGCGGGCAGAGTTTAGCCCTGCCCAGTTCTCTGGCCCTAAGAAGATCAACCTG AACCACTTGTTAAATTTCACCTTTGAACCACGTGGCCAGGCAGGTCACTTTGAAGGCAGTGGGCATGGCG GCTGGGGGAAGAGAAACAAGTGGGGACACAAGCCTTTCAACAAGGAGCTCTTTCTACAGGCCAACTGCCA ATTTGTGGTGTCTGAAGACCAAGACTACGCAGCTCACTTTGCTGACCCTGACACCTTAGTCAACTGGGAC TTTGTGGAACAAGTGCGCATCTGTAGCCATGAAGTGCCATCTTGCCCAATATGCCTCTATCCACCTACTG CGGCCAAGATAACGCGCTGTGGACACATTTTCTGCTGGGCATGCATCCTGCACTATCTTTCACTCAGTGA GAAAACCTGGAGCAAATGTCCCATCTGCTACAGCTCTGTGCATAAGAAGGATCTCAAGAGTGTTGTTGCC ACAGAGTCTCGACAGTATGCTGTGGGGGACACCATTACCATGCAGCTGATGAAGAGAGAGAAGGGAGTGT TGGTGGCCCTGCCCAAATCCAAGTGGGTGAATGTAGACCATCCTATTAACCTAGGAGATGAGCAGCTCAG CCAGTATTCCAAGCTGCTGCTGGCCTCGAAGGAGCAGGTGCTGCACCGGGTAGTTCTGGAGGAGAAAGGA GCGCTGGAGCAGCAGCTGGCAGAGGAGAAGCACACTCCTGAGTCCTGTTTCATTGAGGCTGCTATCCAGG AGGTCAAGATTCGGGAAGAAGCTCTGTCAGGAGTGGCTGGAGGCGGAGGGGAGGTCACTGGTGTTGTGGC TGCTCTGGAACACCTGGTGCTGATGGCTCCCCTGGCCACGGAGTCTGCTTTCCAACCCAGGAAGCAGGGT GTGCTGGAGTATCTGTCTGCTTTTGATGATGAAGCTGCACAAGTTTGTTCTCTGGACCCCCCTGGTCCTC TTGCCCTCCCTTTGGTAGAAGAGGAGGAAGCAGTGTCTGAGCCAGAAGCCTGTGAGGACGCAGAGGTAGC CGATGACAGTCTGGGAGAGGGGACTGTTGGTCCTGAGATGAGCCAGGAGGAGCCCATCACTAAGCCAGGC TTCACCCAGCTCAGCAGCTCTCCGTGTTACTACTTCTACCAGGCGGAAGATGGACAGCACATGTTCCTGC ACCCTGTCAACGTGCGCTGCCTTGTGCGGGAGTACGGCAGCCTGGAGCAGAGCCCAGAGAAGATCTCAGC AACCGTGGTAGAGATTGCTGGGTATTCCATGTCTGAGGATGTTCGGCAGCGTCATAGGTATCTCTCTCAC TTGCCACTCACCTGTGAGTTCAGCATCTGTGAACTGGCTCTGCAGCCTCCTGTGGTCTCTAAGGAAACCC TAGAGATGTTCTCAGATGACATTGAGAAAAGGAAGCGTCAGCGCCAAAAGAAGGCTCGCGAGGAGCGTCG CCGAGAGCGCAGAATTGAGATGGAGGAGAACAAGAGACAGGGCAGGTATCCCGAGGTCCACATTCCCCTC GAGAACCTACAGCAGTTTCCGGCCTTCAATTCCTATACCTGTCCCTCTGATTCTGCTTTGGGTCCCACCA GCACTGAGGGCCATGGGGCCCTATCCCTGTCTCCTCTGAGCAGAAGTCCAGGCTCCCATGCAGACTTTCT GCTGACCCCTCTGTCACCCACTGCCAGTCAGGGCAGTCCTTCCTTCTGCGTTGGGAGTCTGGAAGACGAC TCTCCCTTCCTTTCCTTTGCCCAGATGCTGAGGGTTGGCAAAGCAAAAGCAGACGGGTGGCCCAAAACTG CTCCAAAGAAAGATGACAATAGCTTGGTTCCTCCCGCCCCTGTGGACAGTGATGGGGAGAGTGACAATTC TGACCGTGTTCCTGTGCCCAGTTTCCAAAATTCCTTCAGCCAAGCTATCGAAGCAGCCTTCATGAAATTG GACACACCAGCTACTTCAGACCCCCTCTCTGAAGACAGAGGAGGGAAGAAAAGGAAGAGACAGAAGCAGA AGCTGCTGTTCAGCACCTCGGTCGTCCACACCAAG ACGCGT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAG GTTTAA Protein Sequence ( show hide ) >MR231185 representing NM_001302448 Red =Cloning site Green =Tags(s) MPQSSPSAAATASDMDKNSGSNSSSASSGSSKGQQPPRSASAGPAGESKPKSDGKNSNGSKRYNRKREPS YPKNENFSNQSRRSNSQKSKTFNKMPPQRGGGSSKPFSSSSNGGRRDEVAEAQRAEFSPAQFSGPKKINL NHLLNFTFEPRGQAGHFEGSGHGGWGKRNKWGHKPFNKELFLQANCQFVVSEDQDYAAHFADPDTLVNWD FVEQVRICSHEVPSCPICLYPPTAAKITRCGHIFCWACILHYLSLSEKTWSKCPICYSSVHKKDLKSVVA TESRQYAVGDTITMQLMKREKGVLVALPKSKWVNVDHPINLGDEQLSQYSKLLLASKEQVLHRVVLEEKG ALEQQLAEEKHTPESCFIEAAIQEVKIREEALSGVAGGGGEVTGVVAALEHLVLMAPLATESAFQPRKQG VLEYLSAFDDEAAQVCSLDPPGPLALPLVEEEEAVSEPEACEDAEVADDSLGEGTVGPEMSQEEPITKPG FTQLSSSPCYYFYQAEDGQHMFLHPVNVRCLVREYGSLEQSPEKISATVVEIAGYSMSEDVRQRHRYLSH LPLTCEFSICELALQPPVVSKETLEMFSDDIEKRKRQRQKKAREERRRERRIEMEENKRQGRYPEVHIPL ENLQQFPAFNSYTCPSDSALGPTSTEGHGALSLSPLSRSPGSHADFLLTPLSPTASQGSPSFCVGSLEDD SPFLSFAQMLRVGKAKADGWPKTAPKKDDNSLVPPAPVDSDGESDNSDRVPVPSFQNSFSQAIEAAFMKL DTPATSDPLSEDRGGKKRKRQKQKLLFSTSVVHTK TR TRPLEQKLISEEDLAANDILDYKDDDDK V
• Oti Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Oti Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• E Coli Selection: Kanamycin (25 ug/mL)
• Restriction Sites: SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Mammalian Cell Selection: Neomycin