SR411927
Pcgf6 Mouse siRNA Oligo Duplex (Locus ID 71041)
Origene Technologies, Inc.
DESCRIPTION
Pcgf6 (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each
DETAILS
- Note: Single siRNA duplex (10nmol) can be ordered.
- Purity: HPLC purified
- Refseq: NM_027654, NR_133574, NR_153762, NM_001360632
- Status: 3 Weeks*
- Summary: Transcriptional repressor. May modulate the levels of histone H3K4Me3 by activating KDM5D histone demethylase. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Within the PRC1-like complex, regulates RNF2 ubiquitin ligase activity.[UniProtKB/Swiss-Prot Function]
- Category: RNAi
- Synonyms: 4933407A11Rik; AI604840; MBLR; Rnf134
- Sequences: Available with shipment
- Stability: One year from date of shipment when stored at -20°C.
- Components: Pcgf6 (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each (Locus ID 71041)Included - SR30004, Trilencer-27 Universal Scrambled Negative Control siRNA Duplex - 2 nmolIncluded - SR30005, RNAse free siRNA Duplex Resuspension Buffer - 2 ml Need an individual siRNA?
- Uniprot Id: Q99NA9
- Availability: 3 Weeks
- Quality Control: Tested by ESI-MS
- Performance Guaranteed: OriGene guarantees that at least two of the three Dicer-Substrate duplexes in the kit will provide at least 70% or more knockdown of the target mRNA when used at 10 nM concentration by quantitative RT-PCR when the TYE-563 fluorescent transfection control duplex (cat# SR30002) indicates that >90% of the cells have been transfected and the HPRT positive control (cat# SR30003) provides 90% knockdown efficiency. For non-conforming siRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the siRNA kit. To arrange for a free replacement with newly designed duplexes, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled siRNA control (quantitative RT-PCR data required).
- Number of Transfections: Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).