Anti-p38 MAP Kinase (Thr-180/Tyr-182), Phosphospecific Antibody

DETAILS

Form: Protein A Purified
Gene: Mapk14
Host: Mouse
Rrid: AB_2235133
Type: Primary Antibody
Clone: M139
Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Target: p38 MAP Kinase (Thr-180/Tyr-182)
Isotype: IgG1
Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Shipping: Blue Ice
Synonyms: MAPK, p38, p38alpha, p38MAPK
Clonality: Mouse Monoclonal
Immunogen: Clone M139 was generated from a phospho-p38α (Thr-180/Tyr-182) synthetic peptide (coupled to KLH) corresponding to amino acid residues around threonine 180 and tyrosine 182 of rat p38α. This peptide sequence is highly conserved in the p38β,g, and d MAPKs, and is identical in human and mouse p38α.
Expiration: After date of receipt, stable for at least 1 year at -20°C.
Specificity: This antibody detects a 38 kDa* protein corresponding to the apparent molecular mass of p38α on SDS-PAGE immunoblots of pervanadate treated human Jurkat and A431 cells, as well as anisomycin treated human HeLa cells.
Applications: WB, ICC
Conjugate Exem: Unconjugated
Physical State: Liquid
Quality Control: Western blots performed on each lot.
Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Molecular Weight: 38
Dilution Range Wb: 1:1000
Immunogen Species: Rat
Dilution Range Icc: 1:250
Species Reactivity: Human, Mouse, and Rat
Target Description: p38 MAP kinase (MAPK), also called RK, CSBP, and SAPK2a, is the mammalian orthologue of the yeast HOG kinase. This family of kinases participates in signaling cascades that control cellular responses to cytokines and stress. Four isoforms of p38 MAPK (α,β,γ,δ) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides, UV light, and growth factors. MKK3 and SEK activate p38 MAPK by dual phosphorylation at Thr-180/Tyr-182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 and to phosphorylate the transcription factors ATF-2, Max, and MEF2. T cells possess an alternative pathway for p38 activation where stimulation of the antigen receptor (TCR) induces phosphorylation of p38 on Tyr-323. This site is required for TCR-mediated phosphorylation of Thr-180 and catalytic activity. Thus, Tyr-323 may also have important roles in regulating p38 MAP kinase pathways.
Uniprot Number Immunogen Species: P70618
Post Translational Modification Type: GOmAb/Phosphorylated
Post Translational Modification Residue: Thr-180/Tyr-182