Hydrogen peroxide (H2O2) is a reactive oxygen metabolic by-product that serves as a key regulator for a number of oxidative stress-related states. It is involved in a number of biological events that have been linked to asthma, atherosclerosis, diabetic vasculopathy, osteoporosis, a number of neurodegenerative diseases and Down's syndrome. Perhaps the most intriguing aspect of hydrogen peroxide biology is the recent report that antibodies have the capacity to convert molecular oxygen into hydrogen peroxide to contribute to the normal recognition and destruction processes of the immune system. Measurement of this reactive species will help to determine how oxidative stress modulates varied intracellular pathways. This Cell Meter™ Hydrogen Peroxide Assay Kit uses our unique OxiVision™ Green hydrogen peroxide sensor to quantify hydrogen peroxide in live cells. OxiVision™ Green is cell-permeable, and generates the green fluorescence when it reacts with hydrogen peroxide. The kit is an optimized 'mix and read' assay format that is compatible with HTS liquid handling instruments.