72060
IL-17RA[Biotin]:IL-17A Inhibitor Screening Assay Kit
BPS Bioscience
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DESCRIPTION
The IL-17RA[Biotin]:IL-17A Inhibitor Screening Assay Kit is designed for
screening inhibitors of IL-17A:IL-17RA interaction. The IL-17RA[Biotin]-IL-17A Binding Assay Kit
comes in a convenient 96-well format, with biotin-labeled IL-17RA, purified IL-17A, streptavidinlabeled
HRP, and assay buffer for 100 binding reactions. The key to this kit is the high
sensitivity of detection of biotin-labeled IL-17RA by streptavidin-HRP. Only a few simple steps
on a microtiter plate are required for the assay. First, IL-17A is coated on a 96-well plate. Next,
IL-17RA is incubated with IL-17A on the plate. Finally, the plate is treated with streptavidin-HRP
followed by addition of an HRP substrate to produce chemiluminescence, which can then be
measured using a chemiluminescence reader.
DETAILS
- Notes: MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED: PBS buffer Luminometer or fluorescent microplate reader capable of reading chemiluminescence Rotating or rocker platform
- Shiptemp: -80°C (dry ice)
- Synonyms: Interleukin 17, Interleukin 17 receptor, CTLA8, IL17
- Warnings: Avoid freeze/thaw cycles.
- Category: Immunotherapy/Assay Kit
- Background: IL-17A is a pro-inflammatory cytokine produced by TH17 cells that plays a key role in inflammation, autoimmunity, and host defense. It has been shown to stimulate the production of pro-inflammatory cytokines, namely IL-6 and IL-8, by synoviocytes. Due to its role as a mediator of inflammation, the IL-17 pathway has become a popular target for treating diseases that have a strong inflammatory component such as psoriasis, arthritis, Crohn's disease, and ankylosing spondylitis.
- References: 1. Iwakura Y, et al. 2008. Immunol Rev. 226:57-79. 2. Bullens DM, et al. 2013. Clin Dev Immunol. 2013:840315. 3. Ley K, et al. 2006. Immunol Res. 34(3):229-42.
- Description: The IL-17RA[Biotin]:IL-17A Inhibitor Screening Assay Kit is designed for screening inhibitors of IL-17A:IL-17RA interaction. The IL-17RA[Biotin]-IL-17A Binding Assay Kit comes in a convenient 96-well format, with biotin-labeled IL-17RA, purified IL-17A, streptavidinlabeled HRP, and assay buffer for 100 binding reactions. The key to this kit is the high sensitivity of detection of biotin-labeled IL-17RA by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, IL-17A is coated on a 96-well plate. Next, IL-17RA is incubated with IL-17A on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.
- Methodology: Chemiluminescent
- Unspsc Code: 41121811
- Unspsc Name: Laboratory microchemistry kits
- Applications: Great for screening small molecules and antibodies that inhibit the binding of IL-17A to IL-17RA.
- Product Type: Assay Kit
- Biosafety Level: Not applicable (BSL-1)
- Related Products: 91014-2, 79572, 79512
- Storage Stability: One year from date of receipt when stored as directed.
- Scientific Category: Cytokine, Immunotherapy, Cell Surface Receptor
- Instructions for Use: See product datasheet for detailed protocol.