50383-M08H
Mouse Trypsin 2 / PRSS2 Protein (His Tag)
Sino Biological
DESCRIPTION
A DNA sequence encoding the mouse PRSS2 (NP_033456.1) precursor (Met 1-Asn 246) was expressed, with a C-terminal polyhistidine tag.
DETAILS
- Tag: C-His
- Purity: > 92 % as determined by SDS-PAGE
- Source: HEK293 Cells
- Species: Mouse
- Storage: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
- Activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Anaspec, Catalog #27096) . The specific activity is >1500 pmoles/min/μg . (Activation description: The proenzyme needs to be activated by enteropeptidase for an activated form)
- Molecule: PRSS2
- Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
- Lead Time: 1-3 days
- Formulation: Lyophilized from sterile PBS, pH 7.5
- Molecular Mass: The recombinant pro form of mouse PRSS2 comprises 242 amino acids and has a predicted molecular mass of 26.2 kDa. The apparent molecular mass of rm PRSS2 is approximately 32 kDa in SDS-PAGE under reducing conditions.
- Reconstitution: A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
- Accession Number: NP_033456.1
- Predicted N Terminal: Phe 16