Anti-CD19 CAR Lentivirus (CD19 ScFv-CD8-4-1BB-CD3ζ)

DESCRIPTION

The anti-CD19 CAR lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. These viruses constitutively express the ScFv portion of anti-CD19 (clone FMC63) linked to 2nd generation CAR (Chimeric Antigen Receptor) containing CD8 hinge, 4-1BB, and CD3ζ signaling domains.

DETAILS

Notes: License Disclosure: Visit bpsbioscience.com/license for the label license and other key information about this product. Troubleshooting Guide: Visit bpsbioscience.com/lentivirus-faq for detailed troubleshooting instructions. For all further questions, please email support@bpsbioscience.com.
Species: Replication Incompetent HIV
Shiptemp: -80°C (dry ice)
Synonyms: Anti-CD19 Lentivirus, CAR Lentivirus, Anti-CD19 Lenti, Lenti, Lentivirus, CD19, CD8, 4-1BB, CD3ζ, Anti-CD19, 78600-1, 78600-2
Warnings: Avoid repeated freeze/thaw cycles. Titers can drop significantly with each freeze/thaw cycle. Biosafety: None of the HIV genes (gag, pol, rev) will be expressed in the transduced cells. Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a Biosafety Level 2 facility. BPS recommends following all local federal, state, and institutional regulations and using all appropriate safety precautions.
Category: CAR-T/Lentivirus
Description: The anti-CD19 CAR lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. These viruses constitutively express the ScFv portion of anti-CD19 (clone FMC63) linked to 2nd generation CAR (Chimeric Antigen Receptor) containing CD8 hinge, 4-1BB, and CD3ζ signaling domains.
Formulation: The lentiviruses were produced from HEK293T cells, concentrated, and resuspended in DMEM. Media Formulation Recommended CD4+CD8+ T Cell Medium: StemSpan SFEM (Stemcell Technologies, #09650) supplemented with 10% heat-inactivated FBS (Life Technologies, #10082147), 1% Penicillin/Streptomycin (Hyclone, #SV30010.01), plus 10 ng/ml IL-2 (BPS Bioscience, #90184).
Supplied As: One vial (50 µl) of anti-CD19 CAR at a titer ≥ 108 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
Unspsc Code: 41106621
Unspsc Name: Virus mediated expression vectors or kits
Product Type: Lentivirus
Biosafety Level: BSL-2
Related Products: 90184-A, 60690, 79714, 79059
Storage Stability: Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the virus at -80°C.
Scientific Category: CAR-T
Instructions for Use: See assay protocol for detailed instructions