Monoclonal Antibody to EpCAM (Clone: VU-1D9)

DESCRIPTION

EpCAM (epithelial specific antigen) is an ~40 kDa transmembrane glycoprotein involved in a number of cellular processes including adhesion, proliferation, maintenance of stemness, migration and invasion. EpCAM is expressed on most, but not all, normal epithelia and their corresponding malignancies. Although the role of EpCAM expression in tumorigenesis remains to be fully elucidated, EpCAM may downregulate immunity and help tumors actively escape from immune surveillance.

DETAILS

Gene: EPCAM
App Id: IHC,FACS,WB
Antigen: EpCAM
Gene Id: 4072
Isotype: Mouse IgG1 Kappa
Format Id: Purified
Clone Name: VU-1D9
Uniprot Id: P16422
Host Animal: Mouse
Clonality Id: Monoclonal
Purification: Protein G Chromatography
Reactivity Id: Human
Product Content: 100 µg in 500 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Alternative Name: EPCAM||GA733-2||M1S2||M4S1||MIC18||TACSTD1||TROP1
Application Note: Immunohistochemical analysis: 1:100-1:200; Flowcytometry analysis: 0.2-0.5 µg/10^6 cells, Western blot analysis: 4-6 µg/ml
Storage Condition: Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
Immunogen Information: The NC1-H69 human small cell lung carcinoma line was used as immunogen to generate the EpCAM antibody. The antibody binding epitope has been mapped to the first EGF-like repeat domain (EGF1) amino acids 27-59 of the EpCAM protein.