DESCRIPTION

• Aa: full length
• Mw: 208 kDa (complex)
• Tags: N-terminal FLAG-tag(p110β), no tag on p85α
• Format: Aqueous buffer solution
• Genbank: NM_006219 (p110β), NM_181523 (p85α)
• Species: Human
• Uniprot: p110ß: P42338; p85α: P27986
• Shiptemp: -80°C (dry ice)
• Synonyms: PIK3CB, p110beta, Phosphoinositide-3-Kinase, Catalytic, Beta, PIK3R1, Phosphoinositide-3-Kinase, Regulatory Subunit 1 (Alpha), GRB1, p85alpha
• Warnings: Avoid freeze/thaw cycles.
• Category: Kinase/Lipids
• References: 1. Marques M. et al., (2008). Mol. Cell. Biol. 28 (8), 2803-2814. 2. Straub A. et al., (2008). Thromb. Haemost. 99 (3), 609-615.
• Description: Complex of N-terminal FLAG-tagged recombinant full-length human p110β (GenBank Accession No. NM_006219), and untagged, recombinant full length, human p85α. (GenBank Accession No. NM_181523). Coexpressed in a Baculovirus infected Sf9 cell expression system. p110β MW=124 kDa, p85α MW=84 kDa.
• Formulation: 25 mM Tris, pH 8.0, 69 mM NaCl, 1.35 mM KCl, 0.025% Tween-20, 3 mM DTT, 50% glycerol, and 0.05 mg/ml FLAG peptide.
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
• Host Species: insect
• Product Type: Protein
• Host Cell Line: Sf9 cells
• Biosafety Level: Not applicable (BSL-1)
• Unit Definition: A unit of p110β/p85α kinase activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3,4,5-Trisphosphate/min at 30°C.
• Assay Conditions: A 25 µl kinase reaction is conducted in a buffer containing 40 mM Tris-HCl (pH 7.4), 20 mM MgCl2, 100 uM ATP, 200 µM PI:PS substrate (BPS catalog #40560) and 110β/p85α enzyme for 15 minutes at 30°C. ATP reduction is detected using ADP-Glo™ Kinase Assay Platform (Promega Corporation, Madison, WI).
• Related Products: 40621, 40641, 40640, 40645, 78487
• Specific Activity: 1023 pmol/min/µg
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Kinase/Lipids