Monoclonal Antibody to Actin, Smooth Muscle (Leiomyosarcoma Marker)(1A4 + ACTA2/791)

DESCRIPTION

Actin is a major component of the cytoskeleton and is present in most cell types. It is highly specific to actin from smooth muscles. This MAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.

DETAILS

Gene: ACTA2
App Id: FACS,IF,IHC
Antigen: Actin, Smooth Muscle (Leiomyosarcoma Marker)
Gene Id: 59
Isotype: Mouse IgG2a, kappa + Mouse IgG2a, kappa
Format Id: Purified
Clone Name: 1A4 + ACTA2/791
Uniprot Id: P62736
Host Animal: Mouse
Clonality Id: Monoclonal
Purification: Affinity Chromatography
Reactivity Id: Human, Rat
Product Content: 100 µg in 500 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Alternative Name: ACTA2||ACTSA||ACTVS||GIG46
Application Note: Flow Cytometry (1-2ug/million cells); ,Immunofluorescence (1-2ug/ml); ,Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes);
Storage Condition: Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
Immunogen Information: N-Terminal decapeptide of alpha smooth muscle isoform of actin and conjµgated to KLH (1A4); Recombinant full-length human ACTA2 protein (ACTA2/791)