The Numacut TEV protease is the first protease platform to produce native proteins. Evolved by applying artificial intelligence and directed evolution, the recognition site from TEV protease (ENLYFQ/S) was broadened to ENLYFQ/X (X: any amino acid, except P). Because of this new recognition site, your target is fused C-terminal to ENLYFQ and is cleaved-off by Numacut TEV protease independently of the N-terminal amino acid. This way your targets are produced natively and are released traceless i.e. from Start-Met, fusion partners, tags, cleavage scars or cloning artefacts. In which cases this might be important? For example, if… … the terminus of your target is functionally important. … the the protein has stability or solubility issues with the additional amino acid. … your R&D/preclinical studies were performed with native proteins, and you do not want to change anything “just” for a more efficient process. … you do not want to take any risks. All beneficial mutations from Numaferm’s TEV protease are conserved, making the Numacut TEV protease the ideal sequence-specific protease platform for native protein production. Our Numacut TEV protease harbors an N-terminal 6x His-Tag for easy removal by immobilized metal-ion affinity chromatography (IMAC).