Skip to Main Content
More Intelligent Procurement, Faster R&D

Go to Main Navigation

MR216348L4

Scientist.com Supplier

Atp8a1 (NM_001038999) Mouse Tagged Lenti ORF Clone

Origene Technologies, Inc.

DESCRIPTION

Lenti ORF clone of Atp8a1 (mGFP-tagged) - Mouse ATPase, aminophospholipid transporter (APLT), class I, type 8A, member 1 (Atp8a1), transcript variant 1

DETAILS

  • Tag: mGFP
  • Accn: NM_001038999
  • Type: Mouse Tagged ORF Clone
  • Refseq: NM_001038999.1, NP_001034088.1
  • Status: 3 Weeks*
  • Symbol: Atp8a1
  • Vector: pLenti-C-mGFP-P2A-Puro
  • Category: cDNA Clones
  • Locus Id: 11980
  • Orf Size: 3492 bp
  • Synonyms: AI481521; AI853962; APLT; Atp3a2; AW743152; AW822227; B230107D19Rik; ClassI
  • Refseq Orf: 3495 bp
  • Uniprot Id: P70704
  • Refseq Size: 8175 bp
  • Availability: 3 Weeks
  • Cytogenetics: 5 C3.1
  • Gene Summary: Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules. In vitro, its ATPase activity is selectively and stereospecifically stimulated by phosphatidylserine (PS). The flippase complex ATP8A1:TMEM30A seems to play a role in regulation of cell migration probably involving flippase-mediated translocation of phosphatidylethanolamine (PE) at the plasma membrane. Acts as aminophospholipid translocase at the plasma membrane in neuronal cells; the activity is associated with hippocampus-dependent learning.[UniProtKB/Swiss-Prot Function]
  • Sequence Data: ORF Nucleotide Sequence ( show hide ) The ORF insert of this clone is exactly the same as(MR216348).
  • Oti Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
  • Oti Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
  • Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
  • E Coli Selection: Chloramphenicol (34 ug/mL)
  • Restriction Sites: SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
  • Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
  • Mammalian Cell Selection: Puromycin