DESCRIPTION

• Mw: 51.9 kDa
• Tag: Myc-DDK
• Accn: NM_032701
• Type: Human Tagged ORF Clone
• Refseq: NM_032701.4
• Status: In Stock*
• Symbol: KMT5C
• Vector: pCMV6-Entry
• Category: cDNA Clones
• Locus Id: 84787
• Orf Size: 1386 bp
• Synonyms: Suv4-20h2; SUV420H2
• Refseq Orf: 1389 bp
• Uniprot Id: Q86Y97
• Refseq Size: 2297 bp
• Availability: In Stock
• Cytogenetics: 19q13.42
• Gene Summary: SUV420H2 and the related enzyme SUV420H1 (MIM 610881) function as histone methyltransferases that specifically trimethylate nucleosomal histone H4 (see MIM 602822) on lysine-20 (K20) (Schotta et al., 2004 [PubMed 15145825]).[supplied by OMIM, Dec 2009]
• Chromatograms: CHROMATOGRAMS Sequencher program is needed, download here.
• Sequence Data: ORF Nucleotide Sequence ( show hide ) >RC203881 representing NM_032701 Red =Cloning site Blue=ORF Green =Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGC C ATGGGGCCCGACAGAGTGACAGCACGAGAACTGTGCGAGAACGACGACCTGGCCACCAGCCTCGTCCTGG ACCCCTACCTCGGTTTCCGCACCCATAAGATGAACGTCAGCCCTGTGCCCCCCCTGCGGCGACAGCAGCA CCTGCGCTCAGCGCTGGAAACTTTCCTGAGGCAGCGGGACCTGGAGGCTGCGTACCGGGCCCTGACGCTG GGAGGCTGGACGGCCCGCTACTTCCAGAGCCGGGGCCCGCGGCAGGAGGCTGCCCTCAAGACCCACGTCT ATCGCTACCTCCGTGCCTTCCTGCCGGAAAGTGGCTTTACCATCCTGCCCTGCACGCGCTACTCCATGGA GACCAACGGGGCCAAGATCGTGTCCACTCGTGCTTGGAAAAAGAATGAGAAGCTGGAGCTGCTGGTGGGC TGCATTGCAGAGCTGCGGGAGGCAGATGAGGGGCTGCTGAGGGCCGGTGAGAATGACTTCAGCATCATGT ACTCAACCCGCAAGCGGAGTGCTCAGCTGTGGCTGGGCCCAGCCGCCTTCATCAACCATGACTGCAAACC CAACTGCAAGTTTGTGCCTGCAGATGGGAACGCAGCCTGCGTGAAGGTGCTCCGGGACATTGAGCCTGGG GACGAGGTGACATGCTTCTACGGCGAGGGCTTCTTCGGCGAGAAGAATGAGCACTGTGAATGCCACACCT GTGAGAGGAAAGGTGAAGGAGCTTTCCGAACCAGGCCTAGGGAGCCCGCGTTGCCACCACGGCCCCTGGA CAAGTACCAGCTGCGTGAGACCAAGCGGCGGCTGCAGCAAGGCCTGGACAGTGGCAGCCGACAGGGCCTG CTGGGCCCTCGGGCCTGCGTGCACCCATCCCCGCTGCGCCGGGACCCATTCTGCGCCGCCTGCCAGCCCC TGCGCCTGCCAGCCTGCAGCGCCCGCCCAGACACCTCACCCCTCTGGCTCCAGTGGCTGCCTCAGCCCCA GCCCCGAGTGCGGCCCCGGAAGCGCCGACGCCCCCGGCCCCGGAGGGCCCCAGTGCTCTCCACCCACCAC GCTGCCCGCGTCTCCCTGCACCGATGGGGAGGCTGTGGCCCCCACTGCCGCCTGCGAGGAGAGGCCCTGG TGGCCCTGGGCCAGCCCCCCCACGCCCGCTGGGCCCCTCAGCAGGACTGGCACTGGGCCCGGCGCTATGG GCTGCCTTACGTGGTGCGTGTGGACCTTCGTCGCCTGGCCCCAGCCCCACCAGCTACCCCAGCCCCTGCT GGGACCCCAGGCCCCATCCTGATCCCGAAGCAGGCCCTCGCCTTCGCCCCCTTCTCCCCACCCAAGCGCC TACGGCTGGTGGTCAGCCACGGCTCCATCGACCTGGATGTCGGCGGTGAAGAGCTG ACGCGT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAG GTTTAA Protein Sequence ( show hide ) >RC203881 representing NM_032701 Red =Cloning site Green =Tags(s) MGPDRVTARELCENDDLATSLVLDPYLGFRTHKMNVSPVPPLRRQQHLRSALETFLRQRDLEAAYRALTL GGWTARYFQSRGPRQEAALKTHVYRYLRAFLPESGFTILPCTRYSMETNGAKIVSTRAWKKNEKLELLVG CIAELREADEGLLRAGENDFSIMYSTRKRSAQLWLGPAAFINHDCKPNCKFVPADGNAACVKVLRDIEPG DEVTCFYGEGFFGEKNEHCECHTCERKGEGAFRTRPREPALPPRPLDKYQLRETKRRLQQGLDSGSRQGL LGPRACVHPSPLRRDPFCAACQPLRLPACSARPDTSPLWLQWLPQPQPRVRPRKRRRPRPRRAPVLSTHH AARVSLHRWGGCGPHCRLRGEALVALGQPPHARWAPQQDWHWARRYGLPYVVRVDLRRLAPAPPATPAPA GTPGPILIPKQALAFAPFSPPKRLRLVVSHGSIDLDVGGEEL TR TRPLEQKLISEEDLAANDILDYKDDDDK V
• Oti Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Oti Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• E Coli Selection: Kanamycin (25 ug/mL)
• Protein Families: Druggable Genome
• Protein Pathways: Lysine degradation
• Restriction Sites: SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Mammalian Cell Selection: Neomycin