Human IGF-1(Insulin-like Growth Factor 1) ELISA Kit

DETAILS

Type: Sandwich
Usage: This ELISA kit applies to the in vitro quantitative determination of Human IGF-1 concentrations in serum, plasma and other biological fluids.
Storage: 2-8℃/-20℃,12 months
Precision: Both intra-CV and inter-CV are < 10%.
Assay Time: 3.5h
Uniprot Id: P05019
Application: ELISA
Sensitivity: 0.94 ng/mL
Abbreviation: IGF-1
Recovery Rate: 80%-120%
Category Id Ii: ELISA Kits
Detection Type: Colormetric
Research Areas: Cancer, Developmental biology, Signal transduction
Target Species: Human
Target Synonym: IGF1;IGFI;IGF-I;IGF1A;IGF-IA;IGF-IB;MGF;Somatomedin C;
Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IGF-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IGF-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IGF-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IGF-1. You can calculate the concentration of Human IGF-1 in the samples by comparing the OD of the samples to the standard curve.
Category Id Iii: Traditional ELISA Kits
Detection Range: 1.56-100 ng/mL
Cross Reactivity: This kit recognizes Human IGF-1 in samples. No significant cross-reactivity or interference between Human IGF-1 and analogues was observed
Tested Sample Types: serum, plasma and other biological fluids