DESCRIPTION

• Aa: 1-306(full length)
• Mw: 36 kDa
• Tags: C-terminal His-tag
• Format: Aqueous buffer solution
• Genbank: 1UK3_B,
• Species: SARS-CoV-1
• Shiptemp: -80°C (dry ice)
• Synonyms: coronavirus, 3C-like protease, SARS protease, SARS-ncov protease, covid, covid-19, covid19, Sars-cov, sars cov , sarscov, Mpro, Main protease
• Warnings: Avoid freeze/thaw cycles.
• Category: Coronavirus/Protein
• References: 1. Zhang, L., et al., Science. 2020 Apr; 368(6489): 409-4122. Hsu, M.F. et al., JBC. 2005 Mar; 280:31257-31266.
• Description: Severe acute respiratory Coronavirus 3C-like protease (SARS-CoV 3CL Protease), GenBank Accession No. 1UK3_B, a.a. 1-306(full length), with a C-terminal His-tag, expressed in an E. coli expression system, MW=36 kDa.
• Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, 3 mM DTT, 200 mM Imidazole
• Supplied As: Aqueous buffer solution.
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
• Product Type: Protein
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: Add 40 µl of 3CL protease diluted in 1x assay buffer. Start the reaction by adding 10 µl of diluted substrate. Immediately place plate in plate reader. Read kinetics for 60 min (λexc=360 nm, λem=460 nm). Total volume is 50 µl.
• Related Products: 79955, 100755, 100739, 100784
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Coronavirus