Anti-Prion Protein (Ser-43), Phosphospecific Antibody

DETAILS

Form: Antigen Affinity Purified
Gene: PRNP
Host: Rabbit
Rrid: AB_10615336
Type: Primary Antibody
Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Target: Prion Protein (Ser-43)
Isotype: IgG
Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Shipping: Blue Ice
Synonyms: PrP, PrPsc, PrPc
Clonality: Rabbit Polyclonal
Immunogen: Prion Protein (Ser-43) antibody was generated from a phospho-peptide that included amino acids surrounding Serine 43 in human prion protein. This sequence has high homology to the conserved site in rat, mouse, and bovine prion protein.
Expiration: After date of receipt, stable for at least 1 year at -20°C.
Specificity: The antibody detects a human recombinant Prion protein after phosphorylation by Cdk5/p25 complex. In addition, the antibody may detect aggregrated forms of prion in human PC3 cells treated with Calyculin A.
Applications: WB
Conjugate Exem: Unconjugated
Physical State: Liquid
Quality Control: Western blots performed on each lot.
Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Molecular Weight: 27
Dilution Range Wb: 1:1000
Immunogen Species: Human
Species Reactivity: Human, Mouse, and Rat
Target Description: Prion related neurodegenerative diseases, called transmissible spongiform encephalopathies, are observed in many animal species. These diseases involve conversion of normal cellular prion protein (PrPc) into a form that is insoluble and resistant to proteases (PrPSc). The protease resistant form can polymerize into fibrils which accumulate in infected tissues and cause cell death and tissue damage. PrPs have an N-terminal signal sequence and a C-terminal linkage to glycosylphosphatidylinositol anchor. The mature protein is a glycosylated protein that associates with cell membranes. Phosphorylation of PrPC at Ser-43 by Cdk5 promotes proteinase K resistance, prion aggregation, and fibril formation in vitro. In addition, Ser-43 phosphorylation is upregulated in scrapie-infected mouse brain relative to controls. Thus, phosphorylation of Ser-43 may be an important mechanism leading conversion of PrPc to PrPSc and the onset of disease.
Uniprot Number Immunogen Species: P04156
Post Translational Modification Type: Phosphorylated
Post Translational Modification Residue: Ser-43