Monoclonal Antibody to Ornithine Decarboxylase-1 (ODC-1)(Clone : ODC1/485)

DESCRIPTION

Recognizes a 53kDa protein, identified as the Ornithine Decarboxylase (ODC-1). ODC is the initial and rate-limiting enzyme in the biosynthetic pathway of polyamines and is involved in the conversion of ornithine to putrescine. The biological activity of ODC-1 is rapidly induced in response to virtually all agents known to promote cell proliferation including hormones, drugs, growth factors, mitogens, and tumor promoters. Reportedly, ODC mRNA levels are elevated in lung carcinomas as well as in colon adenomas and carcinomas. ODC activity in colorectal carcinomas is greater than those in adenomas and normal mucosa.

DETAILS

Gene: ODC1
App Id: WB,FACS,IF,WB,IHC
Antigen: Ornithine Decarboxylase-1 (ODC-1)
Gene Id: 4953
Isotype: Mouse IgG1, kappa
Format Id: Purified
Clone Name: ODC1/485
Uniprot Id: P11926
Host Animal: Mouse
Clonality Id: Monoclonal
Purification: Affinity Chromatography
Reactivity Id: Human, Rat
Product Content: 100 µg in 500 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Alternative Name: ODC1
Application Note: Western Blot (0.5-1ug/ml); Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Western Blot (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes);
Storage Condition: Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
Immunogen Information: Recombinant human ODC-1 protein