DESCRIPTION

• Aa: 1-321
• Mw: 63.3 kDa
• Tags: N-terminal GST-tag
• Format: Aqueous buffer solution
• Genbank: NM_011201.3
• Species: Mouse
• Shiptemp: -80°C (dry ice)
• Synonyms: PTP1B, PTPN1, Protein tyrosine phosphatase 1B
• Warnings: Avoid freeze/thaw cycles.
• Category: Protein Phosphatase/Non-Receptor
• Background: Protein tyrosine phosphatase nonreceptor, type 1 (PTPN1) is the founding member of the protein tyrosine phosphatase (PTP) family. PTPN1 catalyze the hydrolysis of the phosphate monoesters specifically from tyrosine residues. PTPN1 is known to be involved in signaling pathways that regulate a variety of cellular processes including cell growth and oncogenic transformation. PTPN1 also has been shown to act as a negative regulator of insulin signaling. PTPN1 has also been reported to dephosphorylate epidermal growth factor receptor kinase, which implicated the role of this phosphatase in cell growth control and cell response to interferon stimulation.
• References: 1. Frangioni et al., Cell, 1992, 68: 545. 2. M. Elchebly et al., Science 1999, 283: 1544.
• Description: Murine recombinant PTP1B Protein tyrosine phosphatase, also known as PTPN1 (Genbank accession No. NM_011201.3), a.a. 1-321 (catalytic domain), with N-terminal GST-tag, MW=63.3 kDa, expressed in an E. coli expression system.
• Formulation: 25 mM Tris-HCl, 75 mM NaCl, pH 8.0, 0.05% Tween-20, 10 mM glutathione, 1 mM DTT and 50% glycerol
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.
• Product Type: Protein
• Purification: Purified using glutathione-agarose beads. The purified GST fusion protein was analyzed on a Coomassie-stained SDS gel.
• Biosafety Level: Not applicable (BSL-1)
• Unit Definition: One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.
• Assay Conditions: The activity of mouse PTP1B was determined using pNPP. Assay conditions: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, 100 mM NaCl, 20 mM pNPP, 5 minute incubation at 30°C, using 2 µg/ml enzyme.
• Related Products: 30009, 30011, 30010, 30019
• Storage Stability: >12 months at -80°C
• Scientific Category: PTP/Non-rec.