DESCRIPTION

• Aa: full length
• Mw: 57 kDa
• Tags: N-terminal His-tag
• Format: Aqueous buffer solution
• Genbank: NM_001626
• Species: Human
• Uniprot: P31751
• Shiptemp: -80°C (dry ice)
• Synonyms: AKT2, PKBβ, PKBbeta, RACbeta, AKT
• Warnings: Avoid freeze/thaw cycles.
• Category: Kinase-Serine/Threonine/Protein
• References: 1. Alessi et al., 1998. Current Biology 8: 69. 2. Cohen et al., 1997. FEBS Lett. 410: 3.
• Description: Recombinant human AKT2 (GenBank Accession No. NM_001626), full length with N-terminal His-tag, MW=60.2 kDa, was expressed in Sf9 insect cells via a baculovirus expression system. The purified AKT2 is activated in vitro using PDK1 enzyme.
• Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 250 mM imidazole, 20% glycerol, and 3 mM DTT.
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
• Host Species: insect
• Product Type: Protein
• Purification: Purified by affinity chromatography
• Host Cell Line: Sf9 cells
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: The enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35, and 2 µM substrate (SER/THR 6, Invitrogen) at 30 °C for 1 hour.
• Specific Activity: 7 pmol/min/µg
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Kinase-Serine/Threonine