E1636Hu
Human G1/S-specific Cyclin-D1, CYCLIN-D1 ELISA Kit
Bioassay Technology Laboratory
DESCRIPTION
Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner.
DETAILS
- Gene Id: 595
- Uniprot: P24385
- Lead Time: 3 days
- Assay Type: Sandwich
- Sample Type: Serum, plasma, cell culture supernates
- Sensitivity: 0.052ng/ml
- Detection Range: 0.1-40ng/ml
- Detection Method: ELISA
- Species Reactivity: Human