Anti-TRPM7 (Extracellular region) Antibody

DETAILS

Form: Antigen Affinity Purified
Gene: TRPM7
Host: Rabbit
Rrid: AB_3068518
Type: Primary Antibody
Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Target: TRPM7 (Extracellular region)
Isotype: IgG
Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Shipping: Blue Ice
Synonyms: TRPM7 TrpC7, LTrpC-7, ChaK1, LTRPC7, TRP
Clonality: Rabbit Polyclonal
Immunogen: TRPM7 synthetic peptide (coupled to carrier) corresponding to amino acids in the extracellular region of human TRPM7. This site is well conserved in rat and mouse TRPM7, but has low homology to other TRPM family members.
Expiration: After date of receipt, stable for at least 1 year at -20°C.
Specificity: This antibody detects a 220 kDa* protein on SDS-PAGE immunoblots of human MCF7, MDA-MB-231, mouse brain, and rat PC12 cells.
Applications: WB
Conjugate Exem: Unconjugated
Physical State: Liquid
Quality Control: Western blots performed on each lot.
Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Molecular Weight: 220
Dilution Range Wb: 1:500
Immunogen Species: Human
Species Reactivity: Human, Mouse, and Rat
Target Description: The transient receptor potential melastatin (TRPM) subfamily of cation-permeable TRP channels is ubiquitously expressed in mammalian tissues. This family includes TRPM1-8. In addition to acting as a calcium-permeant channel, TRPM6 and TRPM7 possess an inherent serine/threonine kinase activity. TRPM7 specifically is involved with cellular magnesium homeostasis and neurotransmitter release. Due to the magnesium inhibition, TRPM7’s ion channel activity is very low. TRPM7 has been implicated in cell proliferation and migration during cancer progression, and its expression levels correlate with prognosis in breast cancer. TRPM7 kinase activation leads to massive autophosphorylation of the C-terminal region, including phosphorylation of Ser-1493, Ser-1513, and Ser-1569. Both Ser-1513 and Ser-1569 phosphorylation is required for kinase activity, and phosphorylation of Ser-1513 may inhibit Caspase-mediated cleavage of the C-terminal tail. Thus, TRPM7 is a multifunctional transmembrane protein with roles in cell signaling, proliferation, migration, and death.
Uniprot Number Immunogen Species: Q96QT4