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32-1744

mSCF Recombinant Protein

Abeomics

DESCRIPTION

Source : Escherichia Coli. Stem Cell Factor Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 165 amino acids & having a molecular mass of 18309 Dalton. The SCF is purified by proprietary chromatographic techniques. Stem cell factor / KIT lig& (SCF) is a cytokine which binds CD117(c-Kit). SCF is also known as 'steel factor' or 'c-kit lig&'. SCF exists in two forms, cell surface bound SCF & soluble (or free) SCF. Soluble SCF is produced by the cleavage of surface bound SCF by metalloproteases.SCF is a growth factor important for the survival, proliferation, & differentiation of hematopoietic stem cells & other hematopoietic progenitor cells. One of its roles is to change the BFU-E (burst-forming unit-erythroid) cells, which are the earliest erythrocyte precursors in the erythrocytic series, into the CFU-E (colony-forming unit-erythroid).

DETAILS

  • Amino Acid: MKEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA.
  • Purification: Greater than 98.0% as determined by:(a) Analysis by SEC-HPLC.(b) Analysis by SDS-PAGE.
  • Product Content: Lyophilized from a concentrated (1mg/ml) solution in water containing 0.02% NaHCO3.
  • Alternative Name: Kit lig& Precursor||C-kit lig&||SCF||Mast cell growth factor||MGF||SF||KL-1||Kitl||DKFZp686F2250||Hematopoietic growth factor KL||Steel factor.
  • Application Note: It is recommended to reconstitute the lyophilized Stem Cell Factor in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. The ED50 as determined by the dose-dependant stimulation of Human TF-1 cell line is < 10 ng/ml, corresponding to a Specific Activity of 1x105 IU/mg.
  • Storage Condition: Lyophilized KIT lig& although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution SCF should be stored at 4°C between 2-7 days & for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.