SEK10333
Human CD50 / ICAM-3 ELISA Pair Set
Sino Biological
Image
DESCRIPTION
The human ICAM3 ELISA Pair Set is for the quantitative determination of human ICAM3
.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for ICAM3coated on a 96-well plate. Standards and samples are added to the wells, andany ICAM3 present binds to the immobilized antibody. The wells are washed and ahorseradish peroxidase conjugated mouse anti-ICAM3 monoclonal antibody is thenadded, producing an antibody-antigen-antibody "sandwich". The wells are againwashed and TMB substrate solution is loaded, which produces color in proportionto the amount of ICAM3 present in the sample. To end the enzyme reaction, thestop solution is added and absorbances of the microwell are read at 450 nm.
DETAILS
- Storage: Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE! Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
- Standard: Each vial contains 40 ng of recombinant ICAM3. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 4 ng/mL is recommended.
- Lead Time: 1-2 weeks
- Application: ELISA
- Assay Range: 62.5-4000
- Specificity: Human CD50 / ICAM-3
- Capture Antibody: 0.5 mg/mL of mouse anti-ICAM3 monoclonal antibody. Dilute to a working concentration of 2 μg/mL in CBS before coating. (Catalog: # 10333-MM02)
- Detection Antibody: 0.5 mg/mL mouse anti-ICAM3 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1 μg/mL in detection antibody dilution buffer before use.