DESCRIPTION

• Form: Antigen Affinity Purified from Pooled Serum
• Gene: EPHB2
• Host: Rabbit
• Rrid: AB_2492088
• Type: Primary Antibody
• Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
• Target: EphrinB (Tyr331)
• Isotype: IgG
• Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
• Shipping: Blue Ice
• Synonyms: CEK5 ligand antibody, EFNB1 antibody, ELK L antibody, ELK ligand antibody, ELKL antibody, EPH related receptor tyrosine kinase ligand 2 antibody, Ephrin B1 antibody, Ephrin B2 antibody, LERK2 antibody
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding the phospho-Tyr331 of chicken EphrinB, conjugated to keyhole limpet hemocyanin (KLH). Note: Chicken Tyr331 is the homolog of mouse and rat Tyr343, human Tyr344 and also Xenopus Tyr324.
• Expiration: After date of receipt, stable for at least 1 year at -20°C.
• Specificity: Specific for endogenous levels of the ~46 kDa EphrinB protein phosphorylated at Tyr331. Immunolabeling is completely eliminated by treatment with λ-phosphatase.
• Applications: WB
• Ncbi Gene Id: 396513
• Conjugate Exem: Unconjugated
• Physical State: Liquid
• Quality Control: Western blots performed on each lot.
• Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
• Molecular Weight: 46
• Production Notes: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
• Dilution Range Wb: 1:1000
• Immunogen Species: Chicken
• Species Reactivity: Mouse and Rat
• Target Description: EphrinB proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development (Flanagan and Vanderhaeghen, 1998; Dufour et al., 2003; Oike et al., 2002). EphrinB molecules expressed at the membrane surface bind to the EphB family receptors on target cells during cell-to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing EphrinB on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of EphrinB is thought to be a critical component of this reverse signaling event (Palmer et al., 2002). Recent work demonstrated that Tyr331 of EphrinB was phosphorylated in HEK293 cells after stimulation by the soluble EphB2 receptor tyrosine kinase (Kalo et al., 2001).
• Expected Reactivity: Bovine, Chicken, Human, Xenopus, Zebrafish
• Uniprot Number Immunogen Species: P28693
• Post Translational Modification Type: Phosphorylated
• Post Translational Modification Residue: Tyr331

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